| Objective:In this paper, we use female precocious puberty rats model which induced danazol, to observe the key molecular Kiss-1 gene derived peptide kisspeptin which regulate the puberty development and its receptor GPR54 (G-protein couped receptor 54) expression at different stages, to discuss the role of kisspeptin/GPR54 in youth start, to observe the effects of kangzao on kisspeptin/GPR54 expression and the function of kisspeptin/GPR54 granules in the treatment of precocious puberty, to make a further understanding of the role of kangzao granules.Kangzao granules are used to treat idiopathic central precocious puberty in girls of 62 cases. We study the relationship of E2, IGF-1 and BMI in precocious puberty girls, as well as kangzao granule regulated Chinese medicine symptoms of precocious puberty girls, to explore the function of kangzao granule. In the mean while, we evaluate the meaning of kangzao granules to treat this disease in clinical economics.Method:(1) The female rats of SD 3 days old who co-feeding with their mother were randomly divided into normal control group A(prepubertal), normal control group B (early youth), experimental group A (early youth), experimental group B (youth medium). Experimental group rats of 5 days old were given subcutaneous injection of 300ug danazol.We used fluorescence quantitative PCR technology to observe Kiss-1/GPR54 mRNA Expression pattern before puberty (23 days after birth), puberty (vaginal opening as a symbol), after puberty (create two rules of the cycle) in hypothalamus of rats..(2) The female rats after 3 days birth who co-feeding with their mother were randomly divided into normal group, model group, triptorelin group, Chinese medicine group (high dose group, middle dose group, low dose group), each group contains 6 rats only. Rats of 5 days birth in model group, triptorelin group and TCM group were given subcutaneous injection of 300ug danazol. The rats of 15days old in triptorelin group begun to use triptorelin 100ug/kg, subcutaneous injection,1 times/day; at the same time, the rats in Chinese medicine group were given 60ml/kg dose one time per day,30ml/kg mid dose,15ml/kg low-dose.While model group rats received normal saline lml/fed only. In Model group, after established the first estrus, the rats were killed during proestrus early process. The rats in other groups as 1:1 ratio at the same time., To calculated ovarian index and uterine index and observed the occurrence of ovarian tissue sections of luteal, we use ELISA method to measure blood E2, LH, IGF-1 levels, and measured Kiss-1, GPR54, GnRH mRNA, GnRH-R mRNA expression changes in hypothalamus by quantitative PCR technology. To discuss the effect of kangzao granules which affect Kiss-1/GPR54mRNA expression and the function of kangzao granules in treatment of precocious puberty.(3) Clinical treatment of idiopathic central precocious puberty girls were randomly divided into 2 groups,62 cases contained in traditional Chinese medicine group,31 cases in western medicine group. Chinese medicine group were treated with kangzao granules one dose per day, flush 100ml in 2 times and served for 6 months continuously; western medicine group were given hydrochloride intramuscular triptorelin, by 60-100ug/kg, for 6 months. To observe the efficacy and advantage before and after treatment in these two groups, and to analyzed the effect of kangzao granules in curing girls'LH,FSH,E2,IGF-1 as well as the relationship of precocious puberty in girls (body mass index) with BMI and E2 (estradiol), IGF-1 (insulin-like growth factor), and kangzao granule to regulate the symptoms of precocious puberty girls with Chinese medicine.To explore the function mechanism of kangzao granules. We evaluate the meaning of kangzao granules to treat this disease in clinical economics.Results:(1) After given subcutaneous injection of danazol to the female rats of 5 days old, the vaginal opening and sexual cycle time are significantly advanced. Ovarian and uterine organ index was significantly increased when comparing with the rats in normal control group A; Puberty of hypothalamic GnRH mRNA expression levels was significantly higher than normal control group A. The expression of Kiss-1mRNA in normal control group A(normal prepubertal) . model group A (early puberty). model group B (mid puberty) were increased gradually,which indicate Kiss-1 mRNA and GPR54mRNA expressions are closely related to growth and development period. At the same time, we observed that when puberty started, the Kiss-1,/GPR54mRNA expression in hypothalamus increased with GnRH simultaneously.(2) We observed that kangzao can be reduced on blood E2, LH (luteinizing hormone), IGF-1 levels on precocious puberty rat model. We observed there was triptorelin group hypothalamus GnRH, Kiss-1 and GPR54 mRNA were significantly decreased by fluorescence quantitative PCR, in Chinese high, medium and low dose group. And there is a significant difference when compared with model group. (P<0.01) It shows that kangzao can regulate expression of Kiss-1, GPR54 and GnRH mRNA in hypothalamic precocious puberty model rats by quantitative PCR technology(3) The TCM clinical efficacy is 83.87%, the western medicine group is 87.09%,there is no significant difference in there two group. The patients serum levels of FSH, LH and E2 levels were decreased significantly, ovary and uterus was retracted, secondary sexual characteristics faded off. The Chinese group can significantly improve the precocious puberty girls body fat symptom, red face, thirst, physical drowsiness, dry stools and other traditional Chinese medicine symptoms.(4) Clinical economics evaluated that Chinese medicine group is better than western medicine group in cost - effective value.
|
PDF Full Text Request