Diabetes Syndrome Characteristics And Effect Of Jiangtang Bushen Recipe On Inflammatory Factors Of Diabetic Rats

BackgroundPrevalence of adult diabetes in our country over the age of20was9.7%, the total number of adults with diabetes in China reached92.4million, approximately90%for type2diabetes mellitus (T2DM).Diabetes has become a major public health problem. Chinese medicine as early as the LingSHU(?)WuBianPian Kidney good disease elimination hate the record,that this disease occurred in the kidney is closely related to, or said that the kidney is a key factor in diabetes incidence.Inflammation hypothesis of diabetes are recognized by more and more industry experts and scholars in recent years in the understanding of Western medicine. T2DM is a chronic, subclinical inflammatory disease,many pro-inflammatory factor, double-stranded RNA and viruses by acting on the IKKβ caused by insulin resistance(IR) and islet β-cell apoptosis,leading to T2DM. IKKβ has become a new pharmacological target of prevention and treatment of T2DM study, inhibition of IKKβ activity can prevent the development of T2DM and its complications.Previous studies showed that traditional Chinese medicine compound the Jiangtang Bushen Recipe has the effect of clearing away heat and efficacy of clinical treatment of T2DM, and confirmed the order to inhibit the inflammatory response. The purpose of this study was to understand the clinical features of type2diabetes and metabolic characteristics,summary of type2diabetes Syndromes TCM syndromes and analysis the correlation of inflammatory markers and other laboratory parameters, to understand the material basis of traditional Chinese medicine syndrome. And on the basis of pre-clinical studies,in-depth to explore the mechanism of the Jiangtang Bushen Recipe treatment of T2DM,start from the source of the role of inflammatory cytokines IKKβ/NF-κ B pathway to observe the the hypoglycemic kidney side pathway links IKKβ expression and activity in rats. The research may clear hypoglycemic specific role of Jiangtang Bushen Recipe, the results will be possible to develop for T2DM inflammation of the biological effects of target prevention and treatment of T2DM in Chinese medicine new drugs,resulting in the objective economic and social benefits.Objectives1.Type2diabetes-related literature to understand the present Western understanding of the doctrine of inflammation in type2diabetes,Systematic reviews of clinical efficacy and safety of the domestic biomedical journals published Bushenhuoxue class traditional Chinese medicine treatment of type2diabetes.2. Clinical surveillance to the clinical features of type2diabetes and metabolic characteristics,and summarize the Syndromes of type2diabetes, understanding the material basis of traditional Chinese medicine with type2diabetes syndrome.3.Experimental study to understand the efficacy of traditional Chinese medicine hypoglycemic kidney side and the specific role of the link.Methods1.Literature researchWestern medicine in recent years,the understanding of type2diabetes inflammation hypothesis literature review, by computer search of the clinical research literature database～the domestic biomedical journals published in May2011for the treatment of type2diabetes Chinese medicine Bushenhuoxue law and its clinical efficacy and safety of the system evaluation.2. Clinical research60cases,the Guangdong Provincial Hospital of Endocrinology outpatient and endocrine ward patients with type2diabetes in March2011to June2011, to collect the patient's general demographic data, and Western-related indicators,indicator of inflammatory factors,complications and concomitant diseases and TCM syndrome score, to EPIdata3.1package to establish a database for statistical analysis, spss17.0.3. Experimental researchPrepared diabetic rats with high fat diet+small dose of streptozotocin by intraperitoneal injection into a mold in rats randomly assigned to the appropriate intervention, and the rats in each group, detect blood glucose levels of rats in each group, serum levels of inflammatory factors, muscle tissue IKKβ mRNA expression and protein levels of InsR phosphorylation of IRS-1tyrosine phosphorylation protein levels, with spss17.0packages for statistical analysis.Results1.The result of clinical research(1)60patients with type2diabetes patients with an average age of61.20±12.95years of age,history of diabetes was8.81±5.97years,body mass index26.24±2.16kg/m2,blood, liver function, renal function, uric acid in the normal range, the TNF-α,IL-6, CRP increased in varying degrees.(2)60cases of type2diabetes patients with complications and diseases associated with the first three is hypertension(40%),dyslipidemia, diabetic neuropathy, diabetic eye disease (account for16.7%)and diabetic nephropathy(10%).(3)In this study, patients with type2diabetes syndrome incidence as follows:kidney deficiency syndrome,Qi and Yin deficiency blood stasis,Liver Qi Stagnation, damp, mind dystrophy, lung and stomach hot,Blood swelter.(4)Canonical analysis of TCM Syndrome Correlation with laboratory indexes:the X5(cholesterol)and Y8(blood stasis)related,X1(BMI) and Y5(hot lung and stomach)between correlation;X6(TNF-alpha)and Y1(kidney deficiency syndrome)between the correlation.2. The result of experimental research(1)The result of fasting blood glucose (FBG) showed that the Jangtang Bushen Recipe can reduce FBG of diabetic rats,the FBG of high-dose of Jiangtang Bushen Recipe group and aspirin group showed a no difference (P>0.05).(2)The Jangtang Bushen Recipe of high,medium and low-dose group can significantly reduce serum TNF-α levels, and significant difference(P<0.05) compared with the aspirin group;the Jangtang Bushen Recipe of high and medium dose group can significantly reduce the serum IL-6level,with the aspirin group and the effect is quite;the Jangtang Bushen Recipe of high, medium and low-dose group could significantly reduce serum CRP levels and a significant difference(P<0.01) compared with the aspirin group (3) The result of Western blotting method showed that protein activity of InsR phosphorylation in muscle tissue of diabetic rats comparison between the model group and control group showed a significant difference(P<0.01), the protein activity of InsR phosphorylation in muscle tissue of diabetic rats comparison between low-dose of Jiangtang Bushen Recipe group and aspirin group showed a no difference (P>0.05),and the2groups are on the same level.The protein activity of IRS-1tyrosine phosphorylation in muscle tissue of diabetic rats comparison between the model group and control group showed a significant difference (P<0.01),the protein activity of IRS-1tyrosine phosphorylation in muscle tissue of diabetic rats comparison with high-dose of Jiangtang Bushen Recipe group, aspirin group and control group showed a no difference (P>0.05),the high-dose of Jiangtang Bushen Recipe group and aspirin group are on the same level (P>0.05)(4) RT-PCR shows that the model group is5.098times higher than blank control group in improving expression of IKKβ mRNA (P<0.01), but the group of Aspirin is2.814times,high-dose group of Jiangtang Bushen recipe is3.246times,medium-dose group of Jiangtang Bushen recipe is3.770times higher than the blank control group in decreasing.compared to model group P<0.01; low-dose group of Jiangtang Bushen recipe is4.974times higher than the blank control group in decreasing.compared to model group P>0.05.Conclusion1.Clinical researchType2diabetes is more common in middle-aged, mostly body obesity, dyslipidemia. Type2diabetes complications and concomitant diseases prevalent hypertension, dyslipidemia, diabetic neuropathy, diabetic eye disease and diabetic nephropathy.Type2diabetic patients serum levels of TNF-alpha, IL-6, CRP, in varying degrees rise.Type2diabetes is deficiency of Ben and repletion of Biao, this deficiency in order to kidney and Qiyinliangxu, mainly standard empirical blood stasis, qi,damp, mainly. In the laboratory markers of type2diabetes and TCM syndrome research found that cholesterol and blood stasis,BMI and lung and stomach hot, TNF-α and kidney deficiency syndrome both correlation.2.Experimental researchJangtang Bushen Recipe significantly reduce fasting blood glucose and serum TNF-alpha, IL-6, CRP levels in diabetic rats,can inhibit muscle tissue of diabetic rats IKKβmRNA expression, increase in muscle tissue of diabetic rats InsR phosphorylation of IRS-1tyrosine phosphorylation in the expression of white eggs, its mechanism may be inhibition of the inflammatory signal transduction pathway with hypoglycemic kidney side, to play to the role of improving insulin resistance and treatment of diabetes.