The Study About The Effect Of CANG LING Powder On Mucosal Immunity And Bacterial Flora In Infant With HRV Diarrhea Of Heat-dampness

ObjectiveA randomized control clinical study of Chinese medicine in infantile rotavirus diarrhea treatment was conducted to evaluate objectively and standardized the effect of cang ling powder on infantile heat-dampness rotavirus diarrhea in terms of clinical syndrome appearance and so on.And observed the effect of Cang Ling powder on mucosal immunity and microecological regulation. to further analyzes the pathogenesis of HRV diarrhea and the possible targets of Cang Ling Powder, to provide scientific basis for the clinical treatment for Human rotavirus (HRV) diarrhea by traditional chinese medicine.MethodsThe clinical study was designed as a prospective, randomized, control trial in target population of6-month to2-year-old infants with HRV diarrhea, who were allocated into Cang Ling Powder group, and smectite group in proportion of1:1with randomizatal method. Clinical symptoms observation and detection of the change of SIgA and microecological regulation in stool between pre/post-treatment and follow-up.Results1. Clinical study of infantile heat-dampness HRV diarrhea1.1Post-treatment total effective rates of Cang Ling Powder group was96.16%and Post-treatment total effective rates of Dioctahedral smectite group is79.17%, there were significant differences when compared the two treatments. Post-treatment total effective rates of Cang Ling Powder group was superior to Dioctahedral smectite group.1.2The antidiarrheal time of Cang Ling Powder group and Dioctahedral smectite group was2.53±1.35days and4.37±1.68days respectively.there was significant difference between the antidiarrheal times of two treatments revealed by statistics analysis. The antidiarrheal time of Cang Ling Powder group was superior to Dioctahedral smectite group.1.3The appetite recovery time of Cang Ling Powder group and Dioctahedral smectite group was3.47±1.52days and5.86±1.81days respectively. there was significant difference between the antidiarrheal times of two treatments revealed by statistics analysis. The appetite recovery time of Cang Ling Powder group was superior to Dioctahedral smectite group.1.4The abdominal distension recovery time of Cang Ling Powder group and Dioctahedral smectite group was3.16±1.54days and5.42±1.93days respectively.there was significant difference between the antidiarrheal times of two treatments revealed by statistics analysis. The abdominal distension recovery time of Cang Ling Powder group was superior to Dioctahedral smectite group.2. Experimental study of infantile heat-dampness HRV diarrhea2.1Content of SIgA were detected in100infants with HRV diarrhea, and the results showed significant difference were revealed between the content of SIgA post-treatment stool specimens of infants in the two groups and that pre-treatment stool specimens; But there was no significant difference of the SIgA content about post-treatment stool specimens in two groups(cangling powder group and smectite group)(P>0.05);There were significant differere between the content of SIgA post-treatment stool specimens of infants in the two groups (cangling powder group and healthy group) specimens.2.2To analyse the content of bifidobacteria, there was no difference in the two groups of both post-treatment stool specimens and pre-treatment stool specimens(P>0.05).2.3To analyse the content of lactobacilli, there was no difference in the two groups of both post-treatment stool specimens and pre-treatment stool specimens(P>0.05).2.4To analyse the content of escherichia coli, there was no difference in the two groups of both post-treatment stool specimens and pre-treatment stool specimens(P>0.05).2.5To analyse the ratio of and escherichia coli, there was no difference in the two groups of both post-treatment stool specimens and pre-treatment stool specimens(P>0.05).2.6To analyse the ratio of lactobacilli and escherichia coli, there was no difference in the two groups of both post-treatment stool specimens and pre-treatment stool specimens(P>0.05).2.7To analyse the content of SIgA and bifidobacteria, there was no correlatons in the two groups of both post-treatment stool specimens and pre-treatment stool specimens(P>0.05).2.8To analyse the content of SIgA and lactobacilli, there was significant correlatons in the two groups of both post-treatment stool specimens and pre-treatment stool specimens(P<0.05).2.9To analyse the content of SIgA and escherichia coli, there was no correlatons in the two groups of both post-treatment stool specimens and pre-treatment stool specimens(P>0.05).Conlusion(1)The study reveal the Cang Ling powder had good effect on HRV diarrhea, it could relieve diarrhea, recover appetite and abdominal distension;So it could decline the course of diarrhea.(2) Through three days, Cang Ling Powder could elevate the content of SIgA in heat-dampness HRV diarrhea infant, there was no significant difference in two groups;Cang Ling Powder had no effect on the bacterial flora content of heat-dampness HRV diarrhea infant, maybe in the forepart of HRV diarrhea, Cang Ling Powder worked throngh other track; or it was limited by the time of collecting stool specimens.(3)Lactobacilli maybe can stimulate the secretion of SIgA...