The Influence Of Buflomedil Pyridoxal Phosphate On The Biological Effect Of Hyperhomocysteinemia In Rats

ObjectiveTo evaluate the effect of buflomedil pyridoxal phosphate on hyperhomocysteinemia(HHcy).Methods40male Sprague-Dawley rats were randomly divided into five groups:buflomedil pyridoxal phosphate (BPP) group; buflomedil+VitB6group; buflomedil group; Met group; the controlled group. At the end of the8th weeks, get blood plasma and unilateral carotid artery to measure the concentration of homocysteine and superoxide dismutase, the protein expression of endothelial nitric oxide synthase.Results1.The Hcy concentration of Met group was significantly higher than controlled group(P<0.01), the Hcy concentration of the BPP, buflomedil+vitamin B6, buflomedil group was lower than Met group(P<0.01), the Hcy concentration of BPP group was significantly lower than buflomedil+vitamin B6group(P<0.01), close to the controlled group(P>0.05).2.The SOD concentration of Met group was significantly lower than controlled group(P<0.01), the SOD concentration of the BPP, buflomedil+vitamin B6, buflomedil group was higher than Met group(P <0.05); the SOD concentration of BPP group was close to buflomedil+vitamin B6group.3. The eNOS expression of Met group was significantly lower than controlled group(P<0.01), the eNOS expression of the BPP, buflomedil+vitamin B6, buflomedil group was higher than Met group(P<0.01), the eNOS expression of BPP group was significantly higher than buflomedil+vitamin B6group(P<0.01), close to the controlled group(P>0.05).4. The eNOS expression was negatively correlated with Hcy concentration.Conclusions1. Buflomedil pyridoxal phosphate, buflomedil+vitamin B6, buflomedil can all decrease plasma Hcy level, and may prevent the negative effect of Hcy to SOD, eNOS.2. Homocysteine can reduce NO production through suppressing the expression of eNOS.3. The influence of buflomedil pyridoxal phosphate on the biological effect of hyperhomocysteinemia was significant, and better than buflomedil+vitamin B6.