Inhibitory Effects Of Sphingosine1-phosphate Receptor2Antagonist On Retinal Neovascularizatiom In Mice

AIM:To investigate the effects of sphingosine1-phosphate receptor2(S1P2R) antagonist on the retinal neovascularization in mice.METHODS:wenty eight5days-old(P5) neonatal C57BL/6J mice were exposed to (75%±2%)oxygen to establish models of vascular proliferative retinopathy. The left eyes of the mice received a vitreous injection of1.5μl100μM JTE-013at its P12after exposed to high oxygen condition.The right eyes with PBS1.5μl injection served as negative control group. The effects of JTE-013on retinal neovascularization were evaluated by counting the endothelial nuclei of new vessels extending "from the retina to the vitreous in histological sections with HE staining. The FITC-dextran staining was used for retinal flatmount to observe the changes of retinal vessels. Neonatal mice fed in normal conditions served as normal controls;and8model mice without JTE-013injection served as control group.RESULTS:The number of the endothelial nuclei of new vessels extending from the retina to the vetreous were significantly less in the left eyes with JTE-013injection than that in the right eyes with PBS injection in the model(3.33±2.57vs19.57±0.42,P<0.001).In the retinal flatm-ounts,the density and abnormality of retinal new vessels were much less in the left eyes with JTE-013injection compared to that of the right eyes with PBS injection in the negative control group and control group.No obvious changes were found in normal group.CONCLUSION:etinal neovascularization can be inhibited,to some extent,by intravitreal injection of JTE-013in this mouse model.