| Part one The protection of extract of Ginkgo biloba preconditioning in rat myocardium after ischemic reperfusion.ObjectiveTo investigate the protection of extract of Ginkgo biloba preconditioning in myocardial ischemia reperfusion injury in the rat.MethodsThree groups (n=20animals/group) of Sprague-Dawley male rats were studied:group S, control rats that received no treatment; group IR, rats treated with NS (1.0ml/kg ip)24h before ischemia; group E, rats treated with extract of Ginkgo biloba (100mg/kg ip)24h before ischemia. Group IR and E were subjected to ischemia by30min of coronary artery occlusion followed by2h of reperfusion. At the end of the reperfusion, myocardial infarct size was measured. The myocardial ultrastructures were observed under the electron microscopy.ResultsEGB caused a significant reduction in infarct size [24.52+/-4.13vs.37.87+/-5.92%(%area at risk) in controls, P<0.05]. The degree injury of Group E was change better than that of Group IR under the electron microscope. ConclusionEGB preconditioning induces the cardioprotection against ischemia reperfusion injury in the rats. Part two Proteomics analysis of extract of Ginkgo biloba preconditioning in rat myocardium after ischemic reperfusion.ObjectiveTo investigate the changes of myocardial protein expression after extract of Ginkgo biloba pretreatment, and to search for the mechanism probably involved in the preconditioning of extract of Ginkgo biloba with proteomics techniques.MethodsProteomics analysis of myocardium protein of EGB pretreatment. The left ventricle tissues of EGB(group E) or IR(group IR) preconditioned rats were sampled for proteomics analysis. The total proteins were extracted and separated by two dimensional gel elecrtophoresis(2-DE), and differential expression protein spots were analyzed with martix-assisted laser desorption/ionization time-of-flihgt mass spectrometry(MALDI-TOF-MS).ResultsAnalysis of2-DE showed that894±18protein spots were in group IR and911±21protein spots in group E, and that the expression of14protein spots were different between the two groups.14protein spots were choosed to do MS analysis, and11proteins were preliminarily identified. These proteins can be classified into four functional groups: metabolism related proteins, anti-oxidant related proteins and others.ConclusionsEGB preconditioning resulted in the changes of protein expression profiles in the myocardial. The differential proteins might function as anti-oxidant reaction and improve the energy metabolism of myocardial to confer cardioprotection. Part three Effect of extract of Ginkgo biloba preconditioning on Cystic fibrosis transmembrane conductance regulator expression during myocardial ischemia-reperfusion in ratsObjectiveTo investigate the effect of extract of Ginkgo biloba preconditioning on Cystic fibrosis transmembrane conductance regulator expression during myocardial ischemia-reperfusion in rats.MethodsThree groups (n=10animals/group) of Sprague-Dawley male rats were studied:group S, control rats that received no treatment; group IR, rats treated with NS (1.0ml/kg ip)24h before ischemia; group E, rats treated with EGB761(100mg/kg ip)24h before ischemia. Group IR and E were subjected to ischemia by30min of coronary artery occlusion followed by2h of reperfusion. At the end of the reperfusion, myocardial infarct size was measured. Cystic fibrosis transmembrane conductance regulator(CFTR) was measured by Western blotting.ResultsEGB761caused a significant reduction in infarct size [24.52+/-4.13vs.37.87+/-5.92%(%area at risk) in group IR, P<0.05]. EGB761treatment induced the higher expression of CFTR in hearts.ConclusionThe EGB761preconditioning attenuates myocardial I/R injury possibly through up-regulating CFTR expression in rats.
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