Studies On Shelterin And TERT Gene Expression With Acquired Aplastic Anemia And The Relationship Between It And TCM Syndrome Type

Objectives: To study the mRNA expression levels of telomere protection proteincomplex shelterin and telomerase reverse transcriptase (TERT) in acquired aplastic anemiapatients' peripheral blood mononuclear cells, explore the relationship between them andacquired aplastic anemia,as well as different TCM syndromes, in order to provide moreaccurate basis of the occurrence,development, treatment and prognosis of aplastic anemia.Methods: Collected Peripheral blood mononuclear cells of60cases with AcquiredAplastic Anemia accordanced with the inclusion criteriamet (in which8cases were acuteaplstic anemia and52cases were chronic aplstic anemia;8cases were acute tuberculosismarrow drying thermy,18cases were deficiency of kidney-yang,19cases were deficiencyof kidney-yin,15cases were deficiency of kidney-yin-and-yang), extraced RNA,andrecorded patients' blood cell count. In addition,collected peripheral blood mononuclear cellof20normal subjects as control group.Used real-time quantitative polymerase chainreaction(RT-qPCR) to detect mRNA expression of shelterin including TRF1,TRF2,RAP1,TIN2,TPP1,POT1and TERT.Analyzed the relationship of each other,and betweenthem and leucocyte count of peripheral blood.Results:1. The expression levels of TRF1and RAP1mRNA in patients with AcquiredAplastic Anemia were higher significantly than control group, there were significantdifferences(TRF1,P＜0.01； RAP1,P＜0.05).The expression levels of TRF2,POT1andTIN2mRNA in patients with Acquired Aplastic Anemia were lower significantly thancontrol group(POT1,TRF2,P＜0.01；TIN2,P＜0.05).2. The expression levels of TRF1and RAP1mRNA in patients with acute aplsticanemia and chronic aplstic anemia were all higher significantly than control group(P＜ 0.05),while POT1and TIN2mRNA were all lower significantly than control group(P＜0.05). The expression levels of TRF2mRNA in patients with acute aplstic anemia werelower significantly than control group(P＜0.05). The expression levels of TIN2mRNA inpatients with acute aplstic anemia were lower significantly than chronic aplstic anemia (P＜0.05).3. The expression levels of TRF1and RAP1mRNA in patients with deficiency ofkidney-yin and acute tuberculosis marrow drying thermy were all higher significantly thancontrol group(P＜0.05). The expression levels of TRF2,TIN2and POT1with deficiencyof kidney-yin were lower significantly than control group(TRF2,POT1,P＜0.05；TIN2,P＜0.01). The expression levels of TIN2,POT1mRNA in patients with acute tuberculosismarrow drying thermy and deficiency of kidney-yin-and-yang were all lower significantlythan control group(P＜0.05).4. The expression levels of TIN2mRNA in patients with deficiency of kidney-yin,deficiency of kidney-yin-and-yang and acute tuberculosis marrow drying thermy werelower significantly than patients with deficiency of kidney-yang(P＜0.05). The expressionlevels of TPP1mRNA with deficiency of kidney-yin were lower significantly thandeficiency of kidney-yang (P＜0.05). The expression levels of TERT mRNA in patientswith deficiency of kidney-yang were higher significantly than patients with deficiency ofkidney-yin, deficiency of kidney-yin-and-yang and acute tuberculosis marrow dryingthermy (P＜0.05).5. There was significant correlation between the expression levels of TIN2mRNA andleucocytes,as well as lymphocytes.There was significant correlation between theexpression levels of POT1mRNA and age. There was significant correlation between theexpression levels of TERT mRNA and red blood corpuscle,as well as hemoglobin.6. There was significant correlation between the expression levels of TERT mRNAand TRF1,TPP1,POT1mRNA. There was significant correlation between the expressionlevels of TRF2mRNA and TPP1,POT1,RAP1,TIN2mRNA. There was significantcorrelation between the expression levels of TPP1mRNA and TERT,TRF2,POT1,RAP1mRNA. There was significant correlation between the expression levels of TIN2mRNAand TRF1,TRF2,POT1mRNA.Conclusions:1. Changes in expression levels of TRF1,TRF2,TIN2,POT1,RAP1 and TERT probablely play a role in the pathogenesis of acquired aplastic anemia. Therewas significant correlation between the expression levels of TERT,TIN2mRNA andleucocytes,as well as lymphocytes.It suggests that TIN2,POT1and TERT is more closelyrelated to the pathogenesis of acquired aplastic anemia.2. The expression levels of TRF1and RAP1mRNA were the highest in patients withdeficiency of kidney-yin,and the lowest in patients with deficiency of kidney-yang. Theexpression levels of TRF2,TIN2,POT1and TPP1mRNA were the highest in patients withdeficiency of kidney-yang,and the lowest in patients with deficiency of kidney-yin.Itsuggested that patients with deficiency of kidney-yin might be more serious lesions,andtelomere loss and apoptosis of hematopoietic cell might be more serious degree thandeficiency of kidney-yang.