Sightseeing Wood Chemical Composition And Anticancer Activity

Tsoongiodendron odorum Chun is a large evergreen tree, belonging to Tsoongiodendron genus, Magnoliaceae family. Now, it has become a peculiar plant of China, being near extinct. It mainly distributes in the sourth of China, such as Guangdong, Guangxi, Hainan Provinces and so on. This plant has low yield of seed, and poor natural renewing, being a rare genus. The genus, being a relatively evolutional genus in Magnoliaceae family is of scientific significance for the studying of catonomic systematics of the family. In the south of China, both stem barks and roots barks of the plant have been antitumour fold medicinal herb. Up to now, there have been no reports on the literature except seeds fatty acids analysis. In the recent thirty years, many kinds of compounds,such as sesquiterpene lactones, alkaloids, phenolic acids and so on, have been isolated from Magnoliaceae family plants. It was found that the sesquiterpene lactones with olefinic bond of C11æ¡Ÿ13 in y-olide ring shows cytotoxitity for human nasopharyugeal carcinoma cells (KB). Proceed from the purpose to save this near extinct plant and to find out fully both the chemical constituents and biological activities of the near extinct plant, and to turn passive protection into initiative protection, we studied systematically both the chemical constituents of T odorum and antitumour activities. Thus, these studies will provide certain scientific basis for the further systematic study of the medical value of this plant. On the basis of systematical consulting the researching literatures of chemical S constituents and biological activities study of Magnoliaceae family plants, and completing literature summary, by means of bioactivity-guiding , antitumour activity screenning assay of multi-tumour cell lines in vitro were made on the root barks , barks, twigs , heart- wood of T odorum Then, the chemical constituents of antitumour activity sections were isolated to rely on different kinds of chromatography techniques, and the tumour inhibition assays of multi-tumour cell lines in vitro were made on bio-active compounds obtained such as costunolide, parthenolide, liriodenine, N-acetylanonaine. The results indicated that above compounds obtained by bioactivity-guiding showed promising anti-tumour activities on the tested tumour cell lines. With both the bioactivity-guiding and the systematical chemical constituents study of root barks ,barks, twigs, heartwood of T odorum , 19 compounds were isolated and identified from above four sections accumulatively, 2 among them are new compounds and others are obtained from T odorum for the first time. Root barks: tumour-inhibition screen assays were made on the petroleum ether section of 95% ethanol extract, it was indicated that HL-60 inhibition rate was 56.87%(inhibition concentration was 100 ii gImL); the assays were made on the EtOAc section of 95% ethanol extract, it was indicated that KB, BGC-823, Bel-7402, HCT-8, HL-60 inhibition rate were 95.00%, 97.24%, 95.10%, 81.59%, 85.11%(inhibition concentration were 10 i.t gImL); the assays were made on the n-BuOH section of 95% ethanol extract, it was indicated that BGC-823, Bel-7402 inhibition rate were 62.20%, 82.99%(inhibition concentration were 10 p gImL) , HCT-8 inhibition rate was 50.83%(inhibition concentration was 100 ii gImL); tumour-inhibition screen assays were made on the EtOAc section of 50% ethanol extract, it were i...