Biological Mechanisms Of Liver Controlling Dispersion Of The Central Nervous Explore From Psychological Stress

1. Research on basic theory of emotional reactions in TCM andpsychological stress in Western MedicineThere are many similarities between emotional reactions and psychological stress considering the concepts, pathogens and the related diseases. There are reliable proofs to study the mechanisms of emotions and emotional diseases from the view of the psychological stress. Not only most of the emotional diseases are related to the Liver (Traditional Chinese Medicine, TCM), but also the methods adopted to treat them according to the theory about liver (TCM) showed the great efficiency. The pathological changes of liver's irregulatory represent the neuroendocrinological disturbance. The traits of psychological stress are series of neuroendocrinological reactions characterized by the activation of hypothalamus-pitutary-adrenal gland axis (HPAA). Therefore, the common basis of the liver irregulatory and the psychological stress is the neuroendocrinological network. The liver plays very important role in regulating psychological stress reactions. It will be feasible to study deeply the modern physiological and pathological mechanisms of Ganzhushuxie (TCM) with the psychological stress theory.The model rats with repeated restraint psychological stress had been established on the base of characteristics of emotional diseases and the methods adopted by the researchers outside or inside. The rules to make the model according with the natural procedure which is similar to the way of emotional diseases will happen then try to avoid the stimulus of physical body factors. The formula of the herbs was made up according to the Liver theory (TCM). We made efforts to probe the neurobiological mechanism of Ganzhushuxie (TCM) by the effects of the formula on the model rats.2. The experimental study2.1 Establishing the model rats with repeated psychological stressThe consecutive dynamic observations of plasma CORT and 3 -EP changes, lasted for 21d , had been carried out. The results of them in the 15th day showed the highestlevel among all of the groups which suggested the intense reaction of psychological stress. That was the reason why the model rats in 15th day was chosen for the further research.Then, the behaviors, serum CORT, plasma ACTH and other physiologic constants (weight, indexes of spleen, thymus, pituitary, and adrenal glands) were observed. The results proved that the model rats characterized by the overreactions of HPAA and showed the signs of depression. The results provided us the further proofs of the feasibility of adopting the model rats of the 15" day to study the psychological stress with the changes of classical indexes. Meanwhile, analyzing all of the results mentioned above including the dynamic changes of plasma CORT and p -EP combined with TCM theory, we made a conclusion that the model is suitable for studying the mechanism of Ganzhushuxie (TCM) in the light of the psychological stress.2.2 The pilot study of nerve center neurobiological mechanism on the Ganzhushuxie (TCM)2.2.1. The detections of plasma, pituitaiy and hypothalamus 3 -EP in each group. Detecting Methods: Radio-immunity analysis.The content of plasma P -EP increased and those of pituitary and hypothalamus decreased significantly. Conclusions:The P-EP in the pituitary and hypothalamus decreased but that of the plasma increased significantly in model group. According to TCM, the pathological mechanism of model rats may be irregnlatory of Liver, and one of the effects of Regulating Liver Therapy was related to enhancing P-EPin hypothalamus.2.2.2. The detections of hypothalamus CRH, plasma P -EP and serum CORT in each group. Detecting Methods: Radio-immunity analysis.Compared with control group, the levels of hypothalamus CRH, plasma P -EP and serum CORT in model rats were all higher. But the levels of CRH in hypothalamus and serum in both Adjusting Liver Group and the Ginsenoside group were lower than those in model group. The plasma P -EP in the Ginsenoside group was higher than...