Acupuncture To Improve The Dynamic Observation Of Focal Cerebral Ischemia In Microvascular Endothelial Cell Function

Research purpose: Cerebrovascular disease with high disability rate, lethality and deathrate endanger human health and life seriously. Cerebral ischemia apoplexy accounts forover half clinical classifying at the cerebrovascular disease,show a tendency to increaseprogressively. The brain capillary and star glue quality cell which enwrap around it under thenormal condition form the permeation extremely low blood-brain barrier(BBB), onlypermit some little molecule to pass; When cerebral ischemia apoplexy happen, theremarkable changes takes place in the permeation of the blood capillary, this kind ofpathology change have direct relation to the change of the function of the brain endothelialcell damaged. Acupuncture can improve the multiple indexes of cerebral ischemia apoplexy modeland participate in the adjustment and recovery of the cerebrovascular endothelial celldamaged, acupuncture 12 well point have great relation to the treatment function andintervene time and course of treatment choose in the local cerebral ischemia model.Continuing to seek the deep mechanism of phenomenon, this research " The dynamicobservation of the acupuncture improve the function of capillary vessel's endothelial cellof local cerebral ischemia model ", adopts middle cerebral artery occlusion method,through the local cerebral ischemia model of rat, detects the change of the blood vesselintegrality marked composition of different time after the lacking blood, and the change ofthe some important expression which adhension molecules and cell factors, attempt todiscuss the inflammation mechanism of the reperfusion of cerebral ischemia and thedynamic effect of the acupuncture improve the function of capillary vessel's endothelialcell of local cerebral ischemia model in the probe level, purposes to prove up the effect ofacupuncture treat the permeation of the BBB and acupuncture protect the endothelial cellin the progress of the cerebral ischemia apoplexy, provides the important feasibility gist ofthe intervene time and the treatment choose in the clinical treatment.Research method: The health old male rats are adopted the MCAO method to create therat's local cerebral ischemia-reperfusion model, examines the expression of the ET-1,Vwfand ICAM-1in the model of rat after cerebral ischemia treatment in 1d,3d,5d,10d with theimmunohistochemical SABC method, marks capillary vessel the vary of the injury andrepair in the progress of the cerebral ischemia-reperfusion; at the same time examines theexpression of IL-1β,IL-6 at each time point with immunohistochemical S-P method,observes the appearance construction of capillary vessel after cerebral ischemia and thevariety regulation of these inflammatory factories expression in capillary vesselendothelial cell.Reseach result:1, The behavioral change of the experimental rat's neural symptom The neural symptom behaviors of every group rat have positive reaction at an hourafter the model created, the sham-operated control group and the control group havesignificance difference (p<0.05), prove the success of the local cerebral ischemia model;英文摘要 5the analyse of every group rat's neural symptom behavior of in the different course ofacupuncture treatment have significance difference,too(p<0.05), prove acupuncture 12well point have remarkable function to the PMCAO rat's neural symptom improvement inthe different course of treatment designed in this experiment.2, the morphologic change of the infarct of the model rat's local brain organizesDissecting model rat, we observe cerebral ischemia apoplexy that lies in the temporal lobeand terminal leaf cortex district, keep the same with brain blood supply area that MCAcontrols; the brain infarct range of 12 well point acupuncture different time group ratobviously reduce than the model group. model rat appear brain hydroncus ,etc. the injuryis mitigated by acupunctur...