| Cerebrovascular disease, well known with its high incidence, high death rate, highrelapse rate and high mutilation rate, is one of the most serious threat of humen being.According to the epidemiological study, incidence of cerebrovascular disease is about140-200/100000 population, average death rate is 140-200/100000 population.In ourcountry the death rate of cerebrovascular disease has bocame the topmost of all the diseasesince 1997. The incidence of ischemic cerebrovascular disease is higher than hemorrhagiccerebrovascular disease, so It is extremely important to study ischemic cerebrovasculardisease. Recently, there a break-through of traditional medical therm in therapy of stroke.Many scholar realized that toxin is one of important concept in the etiology of traditionalChinese medicine. Many evidence suggest toxin affects directly pathological changes andprognosis in acute apoplexy. Some researcher suggest the dogenous heat- toxin is the keyfactor leading to apoplexy. So prescription in clearing away heat and detoxification maybecome hot spot in treating apoplexy. Huang Lian Jie Du Tang, well known as a famous prescription in clearing away heatand detoxification, is composed with coptis root, baikal skullcap root, amur corktree barkand cape jasmine fruit. Recently recearcher of chinese and Japanese found that it hassatisfactory curative effect on ischemic stroke ,which show its good prospect. By usingPatterns and method of traditional medicine active fraction, we prepared the Huang LianJie Du Tang Active Fraction (HLJDTAF) and take a systematic research on it inpharmacology.1 Protective effect of HLJDTAF on experimental cerebral Ischemia In rat. After middle cerebral artery thrombosis (MCAT) in rats, brain infarction andneurologic symptoms occurred. The protective effects of HLJDTAF on rats subjected toMCAT induced with FeCl3 were observed in the experiment. Results showed thatHLJDTAF0.6,0.3,0.15g·kg-1 reduced infarct size by 49.7%,70% and 42.1% respectively,HLJDTAF 0.6,0.3,0.15g·kg-1 attenuated the neurologic symptoms of rats after focalcerebral ischemia. This suggested that HLJDTAF had the treatment effect on cerebralischemic injury. The model of multiple cerebral infarction (MCI) in rats was produced by injectionwith the homologous embolus through unilateral carotid artery . The morphology of theABSTRACT 7brain was observed with HE stains. It was found that the neuronal morphology wasdamaged, there are extensive and multiple foci of infarction in the encephalon and neuronloss was detected in the cerebral 24h after injection. HLJDTAF0.6,0.3,0.15g·kg-1 couldprevent morphology changes induced by MCI . Common carotid artery occ1usion (CCAO) rats was used to analyse the effect ofHLJDTAF on blood brain barrier (BBB) permeability. Results showed that HLJDTAF0.3,0.15g·kg-1 cou1d significant1y reduce the permeabi1ity for Evance b1ue (EB) ofCCAO rats (P<0.05,P<0.01,compared with vehicle). Common carotid artery occlusion and reperfusion repeatedly(CCAORR) in mice wereused to test effect of HLJDTAF on gasp time, brain exponential and water content of brain.Results showed that HLJDTAF0.86,0.43 and 0.22g·kg-1 cou1d significantly prolong theshorten gasp time of CCAORR mice, 0.86 and 0.43g·kg-1 reduce the brain exponential anddecrease the water content of brain(p<0.05;p<0.01 compared with vehicle).2. Protection action of HLJDTAF on the hypoxia mice By the ways of oxygen lack in normal atmosphere, NaNO2 poisoning and decapitationof mice, the effect of HLJDTAF on the hypoxia were observed. The result shows thatHLJDTAF0.86, 0.43, 0.22g·kg-1 can significantly prolong the living time of normalatmosphere, NaNO2 poisoning and decapitation of mice(p<0.05, p<0.01 vs control). Thissuggest that HLJDTAF have certain protective action for hypoxia mice.3. The Effect of HLJDTAF on Acidosis, Energy Metabolism and Free radicalMechanism...
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