Compound Flowers Breeze Vine Extract Against Acute Liver Injury And Mechanism Of Action Of Inflammatory Intervention

Liver injury can be caused by many factors, including trauma, chemical materials (mostly drugs or alcohol) and hepatic virus, etc. The most common cause is hepatitis virus. HBV infection rate is high in our country. According to the epidemiologic investigation on hepatitis serum in 1992 to 1995, the HBsAg rate arrive 9.75%, this means about 1.2 hundred million people have HbsAg in serum. So it is significant to investigate the protection effect of traditional Chinese medicine and the anti-inflammation mechanism. In recent years, it is proved that hepatitis is related with not only virus, but also the body immune system. The histological sign of inflammatory include leukocyte infiltration, cytokines, inflammatory mediums and transcription factors, etc.Sabia. parviflora Wall. ex Roxb mainly grow in Yunnan, Guangxi, Guizhou province. The research materials in before show that it has perfect effect on hepatitis A and hepatitis B. The effect of decreasing ALT, AST and T-Bil is even better. But the concerned pharmacological materials are quite few. In this paper, Parviflora compond was mainly composed of Sabia. parviflora Wall. ex Roxb. By the means of biochemical assay, enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC) and molecular biology, effects of Parviflora compond on the inflammatory reaction in acute liver injury models were studied from liver tissue damage, leukocyte infiltrate, cytokine expression and transcription factors activation, etc. The chief results are as follows.Part One : Protective effects of Parviflora compond on acute liver injury models1 Protective effects of Parviflora compond on liver injury models induced by CCL4 and ANIT in miceOBJECTIVE: To study the effects of Parviflora compond on serum ALT, AST, ALP and T-Bil in liver injury models im mice induced by CCL4 and ANIT. METHODS:The two acute liver injury models were established by CCL4 ip and ANIT ig. Serum ALT, AST, ALP, T-Bil was determined. RESULTS:Serum ALT and AST increased in both models. ANIT ig 48 hours later, serum ALP and T-Bil obviously increased. Parviflora compond (6.5g/kg, 3.3g/kg, ig) can significantly decrease serum ALT level in mice of CCl4 liver injury model. Parviflora compond (13g/kg, 6.5g/kg, 3.3g/kg, ig) can decrease serum ALP and T-Bil level in mice of ANIT liver injury model. CONCLUSION:Parviflora compond have...