Alveolar Macrophages In Elderly Patients With Multiple Organ Failure Initiated By Lung

ObjectiveLung injury followed by infection or SIRS is implicated as a prime initiating event in the mechanism of multiple organ failure in the elderly. Several evidences indicated that alveolar macrophages (AM) are involved in this prime event. Our study was to evaluate the difference of macrophages apoptosis between adult and aged models of zymosan-induced MODS in rats.MethodsAM was enriched from bronchi-alvelar lavage fluid (BALF) in the rats with zymosan-induced MODS and was cultured in vitro for 20 hours. (1) Apoptosis of AM was mearured by propidium staining and flowcytometry. (2) Intracellular calcium of AM was detected by Fluo-3 staining and flowcytometry. (3) Mitochondrial △ ψ mof AM was mearured by Rhodamine 123 staining and flowcytometry.Results(1 )The percentage of AM apoptosis(AM APO%) in controls of aged rats(12.7±3.3%) and adult rats (12.1 ± 2.7%) was lower compared with models. In the models AM APO% of aged rats(43.4 ± 8.4%) was higher than that of adult rats (24.2 ± 3.0%), and their difference was significantly (P<0.01) .(2) With increasing of zymosan administration, APO% and intracellular calcium increased , but Mitochondrial △ ψ m decreased.Conclusion (1) AM APO% of aged rats increased in the models of MOFE. It may be associated...