Propofol In Primary Cultured Neonatal Rat Hippocampal Neurons Hypoxia-reoxygenation Injury

Objective: To establish a primary culture method for newborn rat hippocampal cells, and investigate the early stage apoptosis and death of cultured nerve cells using Annexin V-PI staining protocol. Method: Newborn Wister rats were cooled, sterilized with alcohol and then decapitated under semi-sterile conditions. The cranium was open and hippocampi were isolated from the cerebral hemisphere under anatomic microscope. Trypsin digestion and mechanical dissociation were adopted to get...