.1, The Mid-point Mutations Of The Oligonucleotide Microarray Detection Of Lung Cancer Research, Selenium Dioxide Induced C-fos Protein Expression In Lung Cancer Cells By The Process Of Change,

Objective: Part of the hot-spot mutations of Rb2/p130 gene was screened in resected lung cancers using oligonucleotide gene chips in order to detect rapid and accurate method in gene diagnosis of lung cancer.Methods: Samples of resected lung cancers were collected and genomic DNAs were extracted. After DNA sequence of Rb2/p130 was analysed, probes and primer were synthesized. Gene chips were made by doting the probes to modified slide. Using primer marked by fluorescence, Exon 21 and Exon 22 PCR amplification of Rb2/p130 were performed, then hybridization of the products of PCR amplification and gene chips were used. Hybridization signal was detected in order to assess hot-spot mutation. Finally sequencing was performed to confirm the hot-spot mutation .Results: Using oligonucleotide gene chips, we screened the Rb2/p130 gene mutation in 30 sample of lung cancer. We conclude that the concentration of PCR production and hybridization temperature are the key factors affecting the results. Of the 30 patients, 16.67% with Rb2/p130 mutations. 4(4/20) mutations happened in adenocarcinoma, 1(1/6) mutations happened in squamous carcinoma. Sequencing map confirmed the mutation of Rb2/p130 in lung cancer sample.Conclusion: Rb2/p130 mutations in samples of resected lung cancers were detected by gene chip, but the percentage of mutation in Chinese lung cancer patient was lower than Occident lung cancer patient. The reasons of low mutation rate in Chinese lung cancer may contribute to unable to detect all the mutation lock too small sample size and the differences between the two human races.