Nicotine On Vascular Endothelial Cells Express Intercellular Adhesion Molecule-1 And Its Transcriptional Regulation And Signal Transduction In Cells

Cigarette smoking is one of the major risk factors in the pathogenesis of some inflamatory diseases such as chronic obstructive pulmonary disease and atherosclerotic vascular disease .However ,the mechanisms of such inflamatory process induced by cigarette smoking is still unknown..It's well known ,leucocyte activation aggregation and adherence to endothelial cells(ECs) may be an essential step in initial inflammatory responses. The adherence of leucocyte to ECs is process is mediated by the expression of adhesion molecules. Intercellular adhesion molecule-1 (ICAM-1) is a principal adhesion molecule on endothelial cells and plays a pivotal role in mediating leukocyte adherence to and migration across the endothelium.Previous studies suggest that smoking could increase the adhesion of leukocytes and ECs. Nicotine is a main constituent of cigarette smoke. The goal of this study is to observe the effect of nicotine on binding of leukocytes to ECs ,clarify mechanisms of nicotine-induced expression ICAM-1 and gain insight into the regulation of ICAM-1 gene expression in ECs treated with nicotine. For this purpose, the following aspects are studied : (1) The effect of nicotine on binding of leukocytes to ECs ;(2) The effect of nicotine on expression of ICAM-1 ;(3)The regulatory effect of promoter sequence on nicotine-induced ICAM-1 gene transcription in ECs;(4) The effect of nicotine on intracellular free calcium concentration and activation of Protein Kinase C (PKC) and Ras protein in ECs; (5) The effect of 764-3,a Chinese medicine monomer ,on above change induced by nicotine.