Predict The Radiosensitivity Of Human Tumor Cell Research

Purpose To study the relationship between radiosensitivity and radiation-induced DNA damage and repair in five human tumor cell lines detected by comet assay.Maerials and Methods Using the alkaline comet assay, a microelectrophoretic technique for detection of visual DNA single strand breaks (SSBs), and the tail moment as the end point, to quantify the DNA SSBs, and to sudy the correlation between DNA damage and the radiation dose in five human tumor cell lines of the high differentiated nasopharyngeal squamous carcinoma cell lines (CNE-1), the esophugeal squamous carcinoma cell line (Ec-109), the lung adenocarcinoma cell line (GLC), the poorly differentiated gastric adenocarcinoma cell line (BGC-823) and the rectal adenocarcinoma cell line (HR-8348) after X-ray radiation administered, compared with the clonogenic assay of every cell lines. Also to determine their, spontaneous DNA repair of SSBs after exposure to 16Gy X-ray radiation in both cell lines of the CNE-1 and the GLC, during the period of detection.Result The amount of DNA SSBs induced by a 4Gy radiation single dose was definitely detected with the alkaline comet assay in five cell lines. This assay presented the different results of DNA damage induced by different radiation doses; their DNA damage was increased following the increase of the dose; and there was indeed a...