Mechanism Fails To Grasp The The Tongfu Method To Treat Ischemic Stroke

Background: Ischemic stroke is one of the dangerous, emergent serious diseases in clinic, its episode has not only complex pathological basis, but also dangerous, various and acute procedure, and its morbidity and mortality increase with age growing old. In our country, with the increasing old population and the coming of senile society, it is important and urgent to study pathogenesis of ischemic cerebrovascular disease and explore effective therapeutic schedule in old-age.Objective: According to the theory of traditional Chinese medicine (TCM) ,the article analyses the episode regularity of ischemic stroke. TCM realizes radical pathogenesis of ischemic stroke in acute stage is upper excess(the rampancy of the invading pathogen). The collection of wind, fire, phlegm and stasis leads to obturation of brain and stroke. The therapeutic method of kaiqiao and tongfuhuazhuo is the key of thrapy in acute stage after cerebral ischemia. This topic thoroughly discusses the favorable water function of Kai Qiao Tong Fu recipe from water volume in the brain tissue, matrix metalloproteninase and so on. This topic discusses activation of the blood and protective function of the neuron about Kai Qiao Tong Fu recipe from CGRP and ET-1. On the other hand the topic discusses the treatment mechanism to remodel the nerve function about Kai Qiao Tong Fu recipe from the expression of nerve nutrition factor.Methods: The hitch long-base method is used to duplicate the model of the rats cerebrum median artery reperfusion again 3h after cerebral ischemia , observing water volume and the change of morphology in the brain tissue. RT-PCR method is used to detect the expression of MMP-2mRNA and MMP-9mRNA of each group after MCAO at different time in focal cerebral ischemia. Radioimmunoassay is used to observe the change of serum ET-1 and CGRP of each group after MCAO at different time. Western blot is used to the protein expression of MMP-9 and VEGF of each group after MCAO at different time.Results: By adopting MCAO in rat models to achieve MCAO 3h/R 24h, the nerve symptom of animal of ischemic stroke is alleviated by Kai Qiao Tong Fu recipe. Kai Qiao Tong Fu recipe can regulate the level of serum ET-1 and CGRP after MCAO, protect the brain cell and the blood vessel endothelial cell, reduce the expression of MMP-2, MMP-9mRNA and the MMP-9 protein after MCAO, enhance the expression of VEGF...