| Smilax glabra Roxb. (Family Liliaceae) is the Chinese medicinal herb called tufuling. It has antipyretic, detoxifying, and diuretic actions. It may be beneficial in treatments for brucellosis, syphilis, furunculosis, eczema, dermatitis, nephritis, cystitis, mercury and silver poisoning. In this thesis, a relatively systematic study on chemical constituents, chemical reference, quality control method and biological activity of Smilax glabra Roxb. was performed.Firstly, a study on chemical constituents of Smilax glabra Roxb. was carried out. 20 compounds were isolated and purified from the EtOH extracts of Smilax glabra Roxb., and 17 of them were identified on the basis of spectral data and physicochemical properties, the structures had been elucidated as follows: quercetin, resveratrol, daucosterol,β-sitosterol, astilbin, naringenin, succinic acid, shkimic acid, taxifoliol, ferulic acid, stigmasterol, (-)-epicatechin, palmitic acid, 5-O-caffeoylshikimic acid, 5-hydroxymethylfurfural, lignoceric acid, nicotinamide. Among the compounds, lignoceric acid was isolated from the plant for the first time. The composition of essential oil of Smilax glabra Roxb. was analyzed by gas chromatography-mass spectrometry(GC-MS). 21 compounds were identified, most of them were aromatics.Secondly, it is for the first time that the preparation method and quality standard of the chemical reference of Smilax glabra Roxb.were investigated. The preparation method developed is simple, and the yield and purification of the chemical reference obtained are higher. The chemical reference of 2 g has been accumulated in the course of study.Thirdly, a study on quality control method of Smilax glabra Roxb. was performed. Extraction methods were evaluated for the extraction efficiency of general flavonoids. In this experiment, three factors were studied by orthogonal design, including ethanol concentration, consumption of solvent, and time of extraction. Then, a method for determining general flavonoids in Smilax glabra Roxb. was developed, it is for the first time that we discovered that selection of astilbin as an index is more rational and scientific in the determination of general flavonoids in Smilax glabra Roxb.. Two bioactive constituents namely astilbin and resveratrol in 10 samples were determined with high performance liquid chromatography. The two constituents were completely separated in 20 min. The chromatographic peak areas presented a good linearity with the concentration for this two constituents. To study and develop the fingerprint of Smilax glabra Roxb. by high performance liquid chromatography (HPLC). The results showed that the fingerprints of the samples from 10 different sources were of high similarity with correlation coefficients above 0.9. Peaks existing in all 10 fingerprints were assigned as"common peaks"for Smilax glabra Roxb.. There are 10"common peaks"in the fingerprint. The components were identified by HPLC-MS and HPLC-1H NMR. By comparing the fingerprints, Smilax glabra Roxb. was well distinguished from the commercial adulterants including Heterosmilax japonica Kunth., Heterosmilax yunnanensis Gagnep. and Smilax China L.Finally, the pharmacological activities of Smilax glabra Roxb. on antibiosis, antioxidation, anti-HBV, anti-SIVmac251 and antitumor were studied. The result showed that the EtOAc fraction had some antibacterial activity on Escherichia coli, Staphylococcus aureus, Pseudominas aeruginosa and Shigella dysenteriae; there was no significant effect in the water extraction and compound astilbin. In antioxidation study, compound astilbin and all the fractions extracted from Smilax glabra Roxb. showed different free radical scavenging activity. All the samples mentioned above had no effect on the hepatitis B virus and HepG2 cells. The study demonstrated that the EtOH extract inhibited proliferation of Hela cells. In anti-SIVmac251 study, the result showed that the EtOH extract and the n-BuOH fraction had inhibitory activities against SIVmac251. The research offered a scientific basis for quality control of Smilax glabra Roxb.. To evaluate the association between its Traditional Chinese Medicine effect and pharmacological activities, the effect of"Clearing heat and Resolving toxin"of Smilax glabra Roxb. was predicted by Bayesian belief network. Finally, the effect of"Clearing heat and Resolving toxin"of Smilax glabra Roxb. was further confirmed by this method.In this thesis, the chemical constituents, chemical reference, quality control method and biological activity of Smilax glabra Roxb. were researched, which provide the scientific basis for the further development of Smilax glabra Roxb..
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