| The research explored the theory basis of Feibitang on treating the pulmonaryfibrosis and its effects to pulmonary fibrosis rats caused by bleomycin.On the basis of literature review and tutor clinical practice experiencesummary, got the idea that the main pathogenesis of the disease is theqi-deficiency and blood stasis, the stasis of phlegm and heat, obstruction of thelung collateral. Making clear that the thought of pulmonary fibrosis according tothe pathological factor and pathology. Setting up the treating principle thattonifying qi and promoting blood circulation, clearing heat and removing thephlegm, expel the obstruction from collaterals. It has satisfied effects in clinic forits strong pertinence and both treating the mark and root.The experimental research observed the height,extracellular matrix,cytokine,morphology and molecular biology of the pulmonary fibrosis rats indifferent course of disease which is 7d,14d,28d induced by bleomycin. Exploringthe probable mechanism of Feibitang in the pulmonary fibrosis treatment.Establishing the pulmonary fibrosis rats mode by the method of bleomycininducement. Choosing 180 SD rats(SPF), height200—250g. Anaesthesia methodwas 1.5% sodium pentobarbital abdominal injection, cutting the neck skin of therats after local sterilization. Blunting peeling off the trachea and injecting thebleomycin A5 into the trachea slowly, then stitch up the wound. 180 SD male rats,height 200±10g, dividing into BLM group(30 rats),sham operated group(30 rats),experimental large,middle,small group(90 rats),positive drugs group(30 rats).Converting the Feibitang dosage between human and animals according to theequivalent dosage. And dividing three different dosage group according to theconcentration, small:middle:large=1:2:4. The concentration of the small dosage group is 5ml/kg, the middle group is 10ml/kg, the large group is 20ml/kg, 6d drugsfor a week. The positive drugs group choosed the common drugs prednisone inclinic, sham operated group and control group were given 2ml normal saline perday. All the group were given the drug after the first day of the success modeestablish of the lung interstitial fibrosis. The six group rats were killed since the 7,14,28 days of the experimental. Getting the serum and the lung of the rats by the1.5% sodium pentobarbital abdominal injection anaesthesia. Observing andrecording the rats height, detecting the amount ofⅢtype collagen, hyaluronicacid and IL-6 in the serum with the method of the radioimmunoassay. Doingpathology detection with the right lung tissue and comparing the degree ofalveolitis and pulmonary fibrosis with the method of HE staining. Observing theexpression of the TGF-β1 in the left lung tissue with the method of immunehistochemistry. Detecting the p38MAPK expression in the lung tissue with themethod of Western blotting.The result showed that the experimental group can restrain thephosphorylation activity of P38MAPK and down-regulate the amount ofⅢtypecollagen hyaluronic acid and IL-6, improve the degree of alveolitis and pulmonaryfibrosis, decrease the expression of TGF-β1 in the lung tissue compared with themode group at all times. The most obvious decreased function of the Feibitang isthe middle-dosage group at 7 days. 14, 28d, the Feibitang function ofmiddle-dosage group is most significant. It indicated that the Feibitang canrestrain the matrix reconstruction in the lung interstitial fibrosis rats significantly.The innovation of the project is exploring the function of Feibitang ininterfering the pulmonary fibrosis. By interfering the P38MAPK transductionpassway, restraining the phosphorylation activity of P38MAPK and down-regulating the amount ofⅢtype collagen hyaluronic acid and IL-6,decreasing the expression of TGF-β1 in the lung tissue. Meanwhile, thisinnovation settled the basis of the further research of the pulmonary fibrosis. |
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