Inhibition Of Protein Tyrosine Phosphatase 1B Promotes Differentiation Of Human Adipose-derived Stem Cell

Objective: to study effects of protein tyrosine phosphatase 1B (PTP-1B) on differentiation of human adipose-derived stem cell(hADSC). Methods: 1. hADSC were detected according to stem cell's characteristics. 2.hADSC differentiation was intervented by UA(PTP-1B inhibitor) and sodium orthovanadate(SV, PTP inhibitor). Oil red O staining and FABP4 mRNA were detected. 3. PTP-1B and IR were extracted from hADSC that incubated with UA to detect PTP-1B activity and lever of phosphorylated IRβsubunit. Expression of PTP-1B, PPARγ, C/EBPαand C/EBPβmRNA were detected from 1st to 10th day of induction. Results: 1.hADSC accorded with stem cell's characteristics. 2. Lipid droplets in UA groups were more and bigger and FABP4 increased according as UA dose. No lipid droplet in SV group. 3. UA inhibited PTP-1B activity, made phosphorylated IRβsubunit, PPARγand C/EBPαexpress higher, and C/EBPβearlier. Conclusions: 1. hADSC were cultured successfully. 2. Inhibiton of PTP-1B enhances insulin signal and expression of PPARγand C/EBPα, and makes C/EBPβexpress earlier, which promote differentiation of hADSC. 3.Inhibition of PTP inhibits differentiation of hADSC.