Study On The Relations Between The Activation Of Glia And Intractable And Persisitent Pain From Chronic Prostatitis.

Background and objectives:The origin of chronic prostatitis is complex, although there are lots of foundational andclinical research, we still do not have the unprecedented progress at present. Therefore itstill lacks the effective treatment now.Because the pain from chronic prostatitis is the mainsymptom, and it affect the patients' quality of lives, so the research about the painmechanism of chronic prostatitis is important.,but there is only little study in this aspect indomestic and foreign so far.In recent years we found that the primary central nervous system was L₅-S₂,and pelvicfloor muscle was spasmed after prostate was stimulated,but the pain from prostate wasproduced not only by spasm of pelvic floor muscle,it was characteristic with referredpain,and found that the substance of pain in prostate retain high after prostatitis wassubsidized.the results may speculated in theory that chronic prostatitis resulted in allergicnerve and inflammation of nerve.but the reason of intractable and persisitent pain fromchronic prostatitis retain explore.The recent years studies about glia indicated that there have many ion channels andreceptors of neurotransmitter in glia cell membrane , which can produce and release thenerve active material to maintain the K⁺ balance in neuron, all of which are playing thevital role in some chronic pain. The pain from chronic prostatitis exactly is a chronic,intractable and persisitent pain which is difficult to cure.Therefore the discussion of therelations between glia activation and the pain from chronic prostatitis hopefully seeksevidence for the chronic prostatitis pain in neuropathology, and can provide new targetand experiment for the clinical treatment.Material and methods:This topic took the grown-up big mouse as the experimental object, and injected the complete Freund's adjuvant and carrageenan in prostate gland to make the model of pain,then we investigate the relations between the activation state of glia in spinal cord and painmaterial such as Substance P, the excitability amino acid,IL-1βby the immunohistochemis-try, western-blot,the radio-immunoassay method,the high perform ance liquid chromatogra-phy. In addition we record the cerebral evoked potentials of the mouse in order to make surethe relations between the activation of glia in the spinal cord and pain from chronicprostatitis, so that we can provide new target clue for the treat the intractable and persisitentpain from chronic prostatitis, Also make a initial experiment clue for the occurrence anddevelopment of inflammatory splanchnodynia in neuropathology research.Results:The main results of research are as follows:1.4 weeks after injecting the complete Freund's adjuvant and carrageenen in prostategland, we could find a great quantity of inflammation cells in the prostate gland.2. The activation of glia in spinal cord of mouse with pain from chronic prostatitis wasobvious, it began in 3 days after makeing the model of pain, and in 10 days it reached thepeak, even after 28days, it still had the activation of glia.3.The activation of glia in spinal cord and the changes of pain material such asSubstance P, the excitability amino acid, IL-1βassumed the synchronized change, meaningThe activation of glia in spinal cord was the most important reasons of the change ofSubstance P, IL-1βand excitability amino acid In the chronic prostatitis.4. Opentofylline, the inhibitor of glia activation may suppressed the activation ofglia ,thus , in the end , it caused pain material of spinal cord such as Substance P, theexcitability amino acid, and IL-1βreduced obviously.5. After taking inhibitor of glia activation, the cerebral evoked potentials changedobviously: the latency period of N1 and P1 became longer markedly in comparison with thepain groups.Conclusions:The confirmed of the activation phenomenon of glia in the the model of mouse withpain from chronic prostatitis ;the activation of glia was the most important reason of thechange of Substance P, IL-1βand excitability amino acid in the chronic prostatitis ;Propen-tofylline, the inhibitor of glia activation could restrained the activation of both astrocyte and microglia, it caused the decreasing secretion of Substance P, IL-1β, excitabilityamino acid,and caused the prolonging the latency period of Cerebral evoked potentials.Prospect:Inhibitor of glia activation will be a new remedial target for intractable and persisitentpain from chronic prostatitis...