The Relationship Between JAM1, Tiam1 And Cell Polarity In The Process Of HMSC Differentiate Into Hair Follicle

Objection: To construct a model of hMSC differentiate into epithelial cells from hair follicle (HF) and induce local skin hair follicle reformation. To detect the roles of JAM1; Tiam1; p-PKCζand PAR3 in the process of hMSC differentiate into epithelial cells. To find the relationships between JAM1; Tiam1 and cell polarition; hair follicle oritention.Methods: 1. Thawing hMSC, undertake cells biological character and pathogen detection, then large scale cultivation of cells for next experiment. 2. Isolation, culture and identification of human dermal papilla cells. 3. Using real-time polymerase chain reaction to detect the uPA expression in epidermal cells after human dermal papilla cells induced. 4. Xenografts were initiated by intracutaneous injection of 2×10~4 hMSC or hMSC with JAM1 RNAi. Haematoxylin & Eosin staining was used to detect hair follicle information in different time. 5. Observation of JAM1, PAR3, p-PKCζand Tiam1 by double immunofluorescense of different stage HF using laser scanning confocal microscopy. Western-blotting was used to detect the expression of JAM1, PAR3, p-PKCζand Tiam1 in different stage HF.Results: 1. Hair follicle formation was detected in local nude mice skin after human mesenchymal cell suspensions intracutaneous injection. 2. In the process of HF formation, it's showed that Tiam1 was expressed in all around cells of HF, however, JAM1, PAR3 and p-PKCζwere selectively expressed and enriched on the top of different development stages of HF. 3. JAM1, PAR3 and p-PKCζwere selectively expressed in the root sheath of mature haif follicle. 4. Dermal papilla cells can induce epidermal cells differentiate into hair follicle.Conclusion: hMSC can induce and participate in the HF formation in nude mice. In the process of HF formation, the expression of JAM1, PAR3 and p-PKCζwere selective. The selective expressions of JAM1, PAR3 and p-PKCζhave close relationship to the growth direction and volume increases of HF. p-PKCζare the regulator of PAR3 and Tiam1. JAM1 acceptance the messengers form the microenvironment of nude mice skin tissue and induce the hair follicle development and growth, Tiam1 is the platform of polarity molecular which play key role in the maintenance of hair follicle epithelial cell polarity.