The Experimental Study On Influence Of The Method Of Invigorating The Spleen And Kidney, Benefiting Qi And Nourishing Yin On The Pertention Abillty Of The Kidney Of 2DM Rats

In recent years, the incidence of the Diabetes mellitus rises gradually in the world, and the harmfulness makes people pay attention to it more and more. The Diabetes nephropathy is one of the familiar capillaries complicationses of the dibaetes.The pathogenesis of DN involves many kinds of factors. The relation among TGFβ1, CTGF, VEGF and nephropathy has already become a focus in international researeh. It has been approved that TGF(31,CTGF plays an important role in the occurrenecen and development of DN.CTGF is the downstream medium of TGFβ1, involve in accelerating cell matrix synthesis function of TGFβ1. VEGF is the specific mitogen of endothelial cell, and it can promote endothelial cell proliferation and neonatal blood vessel formation, increase vaseular pemeability.VEGF is the central reason that cause microproteinuria during earlier period of DN, and participates in the renal glomerulus selerosis formation with other factors.Objeetive:To observe the therapeutical effect of strenthenin spleeng, supplementing kidney, supplementing qi and nourishing yin on DN rats and discuss the nosogenesis of DM, and then to bring to light about mechanism of action of the traditional Chinese medicine.Methods:The model rats were established with injection STZ and high ealoriediet. Kinds of examination methods were applied, including biochemistry, light microscope, electron microscope, immunohistochemistry, RT-PCR. The invatigated items are: (1) general condition; (2) 24-hoururinaryalbumin(UAlb);(3) Scr,BUN; (4)BW/ RW; (5) the expression of TGFβ1, CTGF, VEGF; (6) the nephridial tissue of pathologieal change and ultrastructural organization.Results: (1)The models of DN rats have been cloned suceessfully; (2) strenthenin spleeng, supplementing kidney, supple- menting qi and nourishing yin, that has good effects on improving general condition and BW/RW, decreasing Scr, BUN,Alb; (3) regulating VEGF; (4) decreasing expression of TGFβ1 mRNA, CTGF mRNA; (5) repairing acinus renis and nephric tubule, improveing hypertransfusion, high percolation, defending renal function.Conclusions: (1) strenthenin spleeng, supplementing kidney, supplementing qi and nourishing yin, that have been determination of treatment based in pathogenesis obtained through differentiation of symptoms and signs of traditional Chinese medicine. It has effective prescription of DN; (2) it has good effects on improving general condition and BW/ RW, decreasing Scr, BUN,Alb, defending renal function, to achieve prevention and cure; (3) restraining the expression of VEGF, TGF-β1 mRNA, CTGFmRNA. To decrease Alb and kidney hypertrophy; (4)repairing acinus renis and nephric tubule, defending renal function. .