The Effect Of Artificial Light On The Eye And Biothythm And Traditional Chinese Medicine Intervention Function

This topic studied the effects of low intensity artificial light on eye and on biothythm, the intervention role of traditional Chinese medicine to light damage to eye and biothythm disorder.Objective:To study of artificial light on the eye and biothythm and traditional Chinese medicine intervention function by rats experiment.To study the effect of different light environment on refractive error and reproductive condition.Methods:The first part:This experiment planed to simulate light environment of the human ordinary room.The SD rats were exposed to low intensity artificial light intermittently for a long time,daylight lamp and the incandescent lamp as experiment lamp.SD rats 45 without any eye diseases were divides into 3 groups randomly,each group of 15.The control group were exposed to natural light.Experimental group were divided into model group and traditional Chinese medicine group.Two experimental group were exposed to artificial light(light:dark=18:6h) which imitated indoor light of human.The rats of medicine group were given traditional Chinese medicine to fill the stomach according to rat reproductive period.After four months, routine inspection,F-ERG and F-VEP,retina histo-pathology inspection,estrogen content were carded throught to every group.Optical parameter was measured to daylight lamp and the incandescent lamp.The second part:We selected the Xinjiang Uigur Autonomous Region Yili kazak to conduct the social investigation research. Kazak who lived in the pastoral area still maintained traditional life style.Most of them used natural illumination.The artificial illumination time was very short.But the city Kazak illuminated mainly by the artificial light.The illumination time were longer than pastoral Kazak.Two groups of Kazak lived in the different light environment,but the original national custom was invariable.So two groups exactly conformed to us to observe the goal.From November,2005 to November,2006,We investigated grown-up Kazak 427 cases(828 eyes),the age 40-60 year old.The pastoral Kazak were 279 cases(546 eyes),the city Kazak 148 cases(282 eyes).There was no limit to occupation and sex.Anyone whose eye disease affected the vision were excluded.All objects were inspected,including the routine inspection,vision and refractive inspection,questionnaire survey. Results:The first part:The eye tissue profile of each group had not seen the obvious difference.ERG and VEP of control group were more typical,but experiment group's were not.In model group,Latent period of Rod-R were prolonged.The amplitude of a-wave and b-wave of Max-R decreased by 54.26%and 65.35%respectively.The amplitude of a-wave and b-wave of Cone-R decreased by 35.76%and38.46% respectively.The amplitude of OS of Ops and N1-P1 of 30Hz Flicker decreased too. The amplitude of ERG in model group were lower than that in control group(P＜0.05).The amplitude of F-VEP in model group were lower than that in the control group(P＜0.05).ERG and VEP of medicine group were similar to model group,and b-wave of Max-R,Cone-R,Ops OS值,30Hz Flicker N1-P1 were lower than that of control group(P＜0.05).The amplitude of F-VEP were lower than that of control group(P＜0.05).The amplitude of b-wave of Max-R in medicine group were higher than model group.Retina was normal in the control group.The cell arrangement of the control group were clear and orderly by optical microscope.The outer nuclear layer were about 10 to 12 layer thickness,while the inner nuclear layer about 4 to 5.The inner plexiform layer were thicker than outer plexiform layer.The ganglion cell were in nerve fiber layer.The entire retina of the model group atrophied by optical microscope.The cell arrangement of the model group were disorderly.The outer and inner node of photoreceptors disappeared.There was no clear line between outer and inner nuclear layer.The nuclear layer reduced to about 3 to 5 layer thickness.The inner plexiform layer were thinner than that of control group.The ganglion cell and nerve fiber layer were swelled.What to see by optical microscope in medicine group were similar to model group.The control group MDA content were lower than that of two experiment group(P＜0.01).The control group SOD content were higher than that of two experiment group(P＜0.01).The medicine group SOD content were higher than that of model group(P＜0.01).SD rats of control or medicine group were quiet while model group were restless and alarmed.After 4 months,estrogen content in model group were higher than other two groups(P＜0.01).Daylight lamp brightness was not stable and contained much ultraviolet rays,but incandescent lamp was not.The second part:The city Kazak vision were worse.19%(54) the vision is 0.4-0.5,21%(59) the vision 0.6-0.8,18%(50) the vision above 1.0.But the pastoral Kazak vision were better.25%(137) the vision is 0.6-0.8,59%(319) the vision above 1.0.The number whose vision more than 1.0 in pastoral Ka7ak were more than city Kazak(P＜0.05).The city Kazak diopter were between 0D and—4.0D.But the pastoral Kazak diopter mainly concentrated between +2.0D and—2.0D,45%(242) 0D,32%(168)≤+2.0D,18%(101)≤—2.0D.Two groups diopters between +2.0D and 0D or less than 3.0 D have obvious difference.The city Kazak mainly were active in the room.The daylight lamp was the main illumination photosource. 71%(105 cases) the artificial light illuminated more than 6 hours.The pastoral Kazak were active mainly in the outdoor.75%(210 cases) the outdoors activity time were above 9 hours by the natural light illumination.87%(242 cases) the artificial light, main incandescent lamp,illumination time only were 1-3 hour.The number of reproduction were decreased.Conclusion:It suggests that low intensity artificial light can injure retinal function and structure over a long period of time.At same time traditional Chinese medicine is helpful to regulate biothythm disorder.Maybe this medicine can protect retina too. But this opinion needs to discuss further.This research provides experimental basis for the improvement lighting installation.It infers that poor light environment is one of very important reasons to cause refractive error.At same time artifical light environment maybe effect reproductive ability.But this opinion needs to discuss further.