Study On Molecule Anti-tumor Mechanisms Of Baicalin

1.Study on the inhibition effect mechanism of baicalin in S₁₈₀ miceObjectives:To study the inhibition effect mechanism of baicalin in S₁₈₀ mice.Methods:The S₁₈₀ sarcomas was transplanted in 60 KM mice,then they were divided randomly into 5 groups to receive different treatment(high,medium and low dose group of baicalin chloroform extract,cyclophosphosphamide group and control group.The inhibition ratios of the tumor,the spleen index and thymus index were calculated out,the activities of NK cell and the contents of TNF-a,IL-2 in serum were detected.And the levels of VEGF protein was assayed with Western blot.Results:Baicalin could increase the levels of the inhibition ratios of the tumor weight,the spleen index and thymus index in mice,and the effects were in a dose dependent manner.The inhibition ratios of tumor in high,medium and low dose group of baicalin were 56.0%,48.0%,36.0%,and the difference was significant compared with the control group(P＜0.05).The research also finds that the difference of the thymus index,the activities of NK cell and the contents of TNF-a,IL-2 in serum were significant in high,medium and low dose group of baicalin comparing with the control group (P＜0.05).Western blot disclosed that bicalin also could markly downregulate the expression of VEGF gene and compared with control group the difference was significant (P＜0.05).Conclusions:The baicalin could suppres mouse S180 tumor growth and increase the activities of NK cell and the contents of TNF-a,IL-2 in serum significantly.It also could regulate the mice immunity and markly downregulate the expression of VEGF gene.2.Study on the apoptosis-inducing effect mechanism of baicalin on HepG-2 cell lineObjective:To study the inhibition proliferation and the apoptosis-inducing effect of Baicalin and the perhaps mechanism.Methods:The HepG-2 cell were cultured in vitro.To detect the change of the correlated index.The antiproliferation and cytotoxic efficiency of Baicalin on HepG-2 cell was assessed with MTT assay.The cell apoptosis effect was observed by inverted microscope,HE staining and scannin electron microscope(SEM).The cell growth cycle and apoptosis ratio were measured by flow cytometry(FCM).The expressions of caspase-3 protein was detected by Western blot.Results:(1) MTT assay disclosed that Baicalin inhibited the proliferation of HepG-2 cell and the effects were in a time-and-concentration dependent manner.(2) Inverted microscope,HE staining and SEM disclosed that Baicalin induced the typical apoptosis change in cell morphology.(3) FCM disclosed that the ratio of apoptosis was significanfly different as compared with that of control group compared with the control group 5.47%,deviation was significant(P＜0.01) in 48 hours.the tumor cells on G2/M stage decrease obviously and on S stage,the cells increase notablely.(4) Immunocytochemistry disclosed bcl-2,p53 were significantly decreased and bax increased.(5) Western blot disclosed that followed by the increase of Baicalin,caspase-3 protein expression was step up gradually.Conclusions:Baicalin could make some effect on inhibiting proliferation and apoptosis- inducing on HepG-2 cell line,perhaps related to activating of Caspase-3.