Fleabane Extract Retinal Protective Effect Of High Pressure Studies On Gene Regulation

Objective:1.To observe the protection of DSX on gene regulation of rat RGCs cultured in vitro with high pressure.2.To observe the protection of DSX on gene regulation of the rat retinal with chronic,moderately elevated intr aocular pressure(IOP).3.Analyze the mechanism of protection of DSX for rat retina.Method:Setting up RGCs high pressure model cultured in vitro with the condition of 80mmHg for 4 hours,then using gene array technology to study the regulation of gene express of rat RGCs on the variation of pressure.2.Adding DSX into RGCs cultured with high pressure,then using gene array technology to observe the effect of DSX on the regulation the gene express of rat RGCs.3.Building the model of ehronicity,moderately elevated intraocular pressure by the method of Akira's,then using gene array technology to study the regulation to gene express of rat retina.4.Giving DSX orally on IOP rat for one month,then using gene array to observe the effect of DSX on the regulation on the gene express of retina.Results:1.There was 108 genes had differential expression between the model group and the control group of RGCs.such as apoptosis gene NGFR,PAWR,S 100B and ESTs was up-regulation among,other genes hand changed including the transmission of nerve impulse, synaptic transmission,cation channel activity,cell-cell signaling,cytoskeletal protein binding and extracellular matrix.2.There was 51 genes had differential expression between the test group and the model group of RGCs.apoptosis inhibiting genes Bcl2al and Sppl were up-regulation and apoptosis gene NGFR was down-regulation among them,other response to external biotic stimulus genes(4) and ATPase activity genes(3) were up-regulation.3.There was 41 genes had differential expression between the positive control group and the model group of RGCs.apoptosis inhibiting genes Hmoxl was up-regulation and apoptosis gene S100B and NGFR was down-regulation among.4.There was 22 genes had differential expression between the model group and the blank group of retinal apoptosis genes NGFR was up-regulation and CRYBB1 was down-regulation among.5.There was 17 genes had differential expression between the test group and the model group of retinal apoptosis genes NGFR was down-regulation and CRYBB3,CRYGD and CRYBB2 were up-regulation among.Conelusion:1.The pressure could down-regulate the genes such as glutamic acid metabolism or transporter,so made high concentration of Glu,then stimulate Ca²⁺ flowing into intra-cellular,high concentration of Ca²⁺ induced signal transduction system producing cascade reaction,apoptosis gene was up-regulated to make RGCs apoptosis.2.DSX can improve energy metabolism,clean Glu for RGCs,then lessen Ca²⁺ inflow,avoiding Ca²⁺ overloading intra-cellular,blocking up cell signal transduction pathway,making apoptosis genes NGFR down regulation.3.DSX play the role as the protectant of retina by up-regulating apoptosis inhibiting genes Bcl2a1 and Spp1 and several genes about crystalline.4.The protection of DSX for retina is multi- target,complex mechanism,and identify.