Study On Injectable Tissue Engineering Bone With Algin-gelatin Complex As Carriers

Object To explore physico-chemical property,biocompatibility and clinical application of injectable tissue engineering bone carried by algin-gelatin complex.Methods Bone marrow mesenchymal stem cells were abstracted from rabbit bone marrow.And then culture,isolation and amplification were performed in vitro. Osteoblasts,drived from Bone marrow mesenchymal stem cells,were measured and observed.Effect of different concentration solution of calcium chloride on osteoblasts was studied.Effect of different matching ratio complex and sterilization methods on complex was investigated.From cytogenetics,safety,and biocompatibility,of complex, and duration of osteogenesis were explored.Results Bone marrow mesenchymal stem cells were induced into osteoblasts. Concentration of Ca²⁺ could influence metabolic activity of osteoblasts.Different matching ratio,temperature and sterilization methods could change physico-chemicai property of complex.Vapor sterilization for powder of algin had little effect on physico-chemical property of complex.Osteoblasts in complex system grew and proliferated in suspension.To the animals,which were transplanted in complex system of gels and cells,aberration of chromosomal pattern did not occur.After transplantation of the gel by injected into subcutaneous tissue,chondrogenesis and osteogenesis happened.And maximum defect of skull was repaired with os cartilaginea.Conclusion Bone marrow mesenchymal stem cells can be induced into osteoblasts in vitro.Different concentration and duration of calcium chloride have effects on osteoblasts.Physico-chemical property of algin-gelatin complex can satisfy requirement of osteoblasts cartier.Vapor sterilization for powder of algin has little effect on physico-chemical property of complex.Complex system has no effect of chromosomal pattern aberration.Using the injectable method heterotopic bone can be applied to repair defect of bone.