The Inhibition Of IL-4 To Cyclooxygenase-2 And Platelet-derived Growth Factor In The Rats Of COPD

Chronic obstructive pulmonary disease(COPD) is a important cause of growing morbidity and mortality in worldwide respiratory system It is characterized by incompletely reversible limited gas flow and gradually decreased lung function. The pathogenesy of COPD has not been fully understood yet. We now generally consider that COPD is a kind of chronic inflammation in its airway and lung parenchyma and lung blood vessel. COPD has increased alveolar macrophage and T lymphocyte,especially CD+8,and neutrophil in the different places of lung. Activated inflammatory cells can release many kinds of mediators. These mediators can destroy lung construction and promote inflammation reaction of neutrophil. Besides inflammation, the imbalance of lung protease and antiprotease and of oxidation and antioxidation also has an important effect in the development of COPD. Its occurrence and development is intimately correlated with various tendency factor, cell, growth factor and enzyme. In our study, we used immunohistochimstry and semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) and western blotting to detect the expression of cyclooxygenase-2(COX-2) and platelet-derived growth factor A(PDGF-A) and platelet-derived growth factor B(PDGF-B) in the lung tissues and its alveolar epithelial cell. We also observed the effect of the cytokine of interleukin 4(IL-4) in our experiment. Finally we investigate the nosogenesis and prevention and cure of COPD. The study consists of three parts as follows: Partâ… The expressions of COX-2 and PDGF-A and PDGF-B in the lung tissues of ratsObjective To explore the expressions of COX-2,PDGF-A and PDGF-B in the lungs of rats of COPD and the effect of dexamethasone in this study.Methods We used male Wistar rats to build up the model of COPD. Twenty-four rats were randomly divided into the control group, the model group and the dexamethasone group. Every group had eight rats. The expressions of COX-2,PDGF-A and PDGF-B in the lung tissue were detected by immunhistochemistry, semi-quantitative RT-PCR and western blotting.Results Compared with the control group,the expressions of COX-2,PDGF-A and PDGF-B in the model group and dexamethasone group were increased significantly. And compared with the model group, those expressions were decreased obviously in the dexamethasone group.Conclusion The expressions of COX-2 and PDGF in the lung tissue of COPD were increased significantly and dexamethasone can decrease those expressions. It also meaned dexamethasone can interfere in the model of COPD.Partâ…¡The inhibition of IL-4 to the form of COPD of smoking ratsObjective To explore the role of IL-4 in the model of COPD and the expressions of cyclooxygenase-2(COX-2) and platelet-derived growth factor(PDGF) in the lungs of rats of COPD.Methods We used male Wistar rats to build up the model of COPD. Forty rats were randomly divided into the control group, the model group, the IL-4a group,IL-4b group and the dexamethasone group. Every group had eight rats.The expressions of COX-2,PDGF-A and PDGF-B in the lung tissue were detected by immunhistochemistry, semi-quantitative RT-PCR and western blotting.Results Compared with the control group, the expressions of COX-2,PDGF-A and PDGF-B in the model group, the IL-4a group, the IL-4b group and the dexamethasone group were increased significantly. And compared with the model group, those expressions were decreased obviously in the IL-4a group, the IL-4b group and the dexamethasone group. Those expressions in the IL-4 group and dexamethasone group had not statistically significant.Conclusion IL-4 and dexamethasone can interfere the establishment of COPD. The expressions of COX-2,PDGF-A and PDGF-B in the lung tissue of COPD were increased significantly and IL-4 and dexamethasone can decrease those expressions.Partâ…¢The expressions of cyclooxygenase-2 and platelet-derived growth factor in alveolar typeâ…¡cells of rats of COPDObjective To study the expressions of COX-2,PDGF-A and PDGF-B in alveolar typeâ…¡epithelial cells of the rats of COPD, and the influence of IL-4 and dexamethasone to those expressions.Methods We used male Wistar rats to build up the COPD model, then we isolated their lungs to primary culture the alveolar typeâ…¡epithelial cells. Rats were randomly divided into the control group and the model group. We used IL-4 and dexamethasone to interfere the cells of the model group. The expressions of COX-2 and PDGF-A and PDGF-B in the lung tissue were detected by immunohistochemistry.Results The expressions of COX-2 and PDGF-A and PDGF-B in the model group were increased significantly. Those expressions in the IL-4 and dexamethasone interfered groups were decreased obviously.Conclusion The expressions of COX-2 and PDGF in alveolar typeâ…¡cells of the COPD lung tissue were increased significantly and IL-4 and dexamethasone can decrease those expressions.