Experimental Study On Intrathymic Inoculation Of Allogeneic Antigen Induces Organization Allograft Immunotolerance In Mice

Objective:To investigate the influence of intrathymical injection with different dose allogenic antigen in induction of sciatic allograft immunotolerance in rats.Methods:Male Sprague Dawley(SD) rats were idvided into 5 groups(ten rats in each group):nerve autograft group,nerve allograft group,low-dose,medial-dose and high-dose MHC antigen injection groups and male Wistar rats(20 rats) were used as transplant donors.Different dose MHC antigen(1.5mg,3mg,6mg/per group) was extracted from Wistar rats splenocytes through destruction,ultrafugation,dialysis and was intrathymically injected into SD recipients rats,a week after the injection,Wistar rats' scaric transplantation was performed.Two weeks after the surgery,lab-tests were examined:nerve electrophysiology,pathology,sIL-2R,mixed lymphocyte culture and delayed type hypersensitivity.Results:Motor nerve conduction velocity in group E was(25.56±4.67)m/s and there was no significant difference compared with group A(p＞0.05),but better than group C(p＜0.05).The detections in pathology and electron microscope confirmed that group E and A were similar(p＞0.05)and better than group C,D and group C,D better than B(p＜0.05).The mixed lymphocyte culture in group E was(0.392±0.096). sIL-2R levels of group E was(0.117±0.050).The delayed type hypersensitivity in group E was(1.34±0.478)mm.Conclusions:Induce specific immunotolerance's effect of intrathymical injection of donor MHC antigen could be improved come up with disparate dose used. Objective:To study the effects of inthathymic inoculation allogenic antigen on the establishment of donor specific bone allograft immunotolerance.Methods:MHC antigens extracted from splenic cells of donor SD mice were intrathymically inoculated to recipients Wistar mice,donor bone was transplanted after 2 weeks.Simultaneous-ly,four control groups were used,such as bone autograft group,bone allograft group,bone freeze thawing allograft group and bone allograft in use of immunosuppres- sant group.At 1,2,4,6 weeks postoperatively,2 animals were sacrificed respectively and the femur and its peripheral tissue was harvested for gross and microscopy observation after symptom and X—ray examination.The ultra microstruc- ture of graft.soluble interleukin-2 receptor,mixed lymphocyte culture and delayed-type hypersensitivity were also detected 4 weeks after the procedure.Results:The test group surpassed bone freeze thawing graft group and bone allograft group and approached to bone autograft group and bone allograft in use of immunosuppressant group.Conclusions:The inthathymic inoculation of donor MHC antigen could induce specific immunotolerance of bone allograft.