In Vivo Study Of Cerebral Hypoxia-ischemia Injury Induced Catecholamine Signal In Striatum And CA1 By Amperometry

BACKGROUND: Treatment of Cerebral hypoxia-ischemia injury isquite difficult in clinical. As a neurotransmitter, dopamine plays a veryimportant role in hypoxia-ischemia brain injury process. There is still notan effective device to detect the changes of dopamine while cerebralhypoxia&ischemia.OBJECTIVE: To establish acute cerebral hypoxia-ischemia injurymodel of rat and detect the hypoxia-ischemia-induced catecholaminesignal(HICS) by carbon fiber microelectrode(CFE)with electrochemicaltechnique in an in vivo model of cerebral hypoxia-ischaemia injury.METHODS: Establish an acute cerebral hypoxia-ischemia injury modelof rat by ligation of lateral common carotid artery and inhalation of 100%N₂. Quantify the catecholamine release in rat striatum and CA1 in vivowith electrochemistry technique(amperometry), and to get the HICS .Verify the stability and reliability of HICS.RESULTS: HICS can be induced by hypoxia-ischemic brain injury invivo and it can be repeated well. The key component of HICS isdopamine.CONCLUSION: a. HICS can be recorded in either striatum or CA1 inrat. HICS in striatum presents monophase, while HICS in CA1 presents diphase. b.HICS is a new stable and reliable signal and can be used to estimate effects of cerebral hypoxia-ischemia injury in vivo.