The Associations Between Uygur, Han APOA5, APOB, CETP Gene And Lipids Of Coronary Heart Disease With Diabetes

Objective:To investigate the Han,Uygur diabetes patients with coronary heart disease,coronary heart disease patients,diabetic patients with blood lipids,blood sugar, blood pressure and other common risk factors for the distribution,at the same time explore the APOA5,APOB,CETP gene polymorphisms with coronary heart disease in diabetes,and coronary heart disease,and diabetic patients distribution characteristics, clear whether there are differences,respectively,to explore and further define APOAS, APOB,CETP gene polymorphisms with the Han,Uygur coronary heart disease,diabetes, coronary heart disease associated with diabetes mellitus.Methods:from January 2008 to October 2008 in the heart center and inpatient endocrine patients as research subjects,266 cases of patients with coronary artery disease,196 cases of male,female 70 cases;one of 154 cases with diabetes,110 cases of male,female 44 cases;122 cases of patients with diabetes alone,one of 84 cases of male,female 38 cases.137 cases of the control group, one of 77 cases of male,female 60 cases.Measurement of height,weight,blood glucose, blood lipids,systolic blood pressure,diastolic blood pressure,blood total cholesterol, triglyceride,high density lipoprotein,low density lipoprotein and other indicators, Calculation of apolipoprotein B/A ratio and Smoking index calculation for different groups to compare,contrast,application of non-conditional Logistic regression analysis of diabetic patients with coronary heart disease,coronary heart disease,diabetes risk factors.At the same time,extracting leukocyte genomic DNA,respectively,by polymerase chain reaction(PCR) amplification and conection enzym method detection of CETP,APOA5,APOB gene polymorphism analysis of the above polymorphisms with coronary heart disease and diabetes incidence relations.Results:1) APOA5 gene 1131T/C sub-type Tip:coronary heart disease with diabetes mellitus group C allele carriers(CC+TC) TG and LP(a) levels were significantly higher than the C non-carriers (TT);CHD group and CHD with diabetes allele frequencies significantly higher in the control group.Diabetes group and the control group C was no significant difference in allele frequencies.Case Uighur groups,no difference between genotype frequencies of the Han,in coronary heart disease with diabetes mellitus group Uygur C allele frequency is higher than the Han in CHD group and diabetes group.2) APOB gene polymorphism and blood lipid target EocRI no obvious relevance,although one of A allele frequencies in theCHDgroup,diabetic control group,but did not reach statistical significance,patients with coronary heart disease in diabetic and control groups the same,A allele frequency and incidence of coronary heart disease there is no consistency,coronary heart disease allele frequency of group A the highest,followed by diabetic group,diabetic patients with coronary heart disease group and the control group;A allele with elevated blood lipids and crown heart disease unrelated to happen,cases and controls the distribution between the A allele frequency was no significant difference.Group A allele carriers of coronary heart disease with diabetes is higher than G allele carriers BMI;in coronary heart disease group and CHD with diabetes group Uygur GA genotype frequency and allele frequency is higher than the Han nationality,and in the group of diabetic there is no difference.3) D442G in the CETP gene among the four groups were found mutation D442G homozygote GG genotype,coronary heart disease group D442G mutation in CETP gene frequency of 4.5%(5/112),coronary heart disease with diabetes mutation rate of 3.9% (6/154),diabetes,mutation rate 4.9%(6/122),while the control group,3.7%mutation frequency(5/136),case and control groups were not statistically significant mutation frequency(P＞0.05).Two groups are consistent with Hardy- Weinberg balance,with a representative group.Conclusion:The high blood lipids,hypertension,high blood sugar, body mass index is higher in diabetic patients with coronary heart disease risk factors of coronary heart disease group similar group of diabetic hypertension,high blood lipids, body mass index increase in risk factors.4) diabetic group,coronary heart disease group, diabetic patients with coronary heart disease group TG,TC,LDL,APOB,blood sugar were higher,reaching statistical difference,P value＜0.05,Between the three age groups, BMI,HDL-C,LDL-C,APOA1,showed differences in smoking,one group of diabetic patients with coronary heart disease higher in the diabetic group and coronary heart disease group,but after no significant differences between the two groups.Coronary heart disease Uighur group HDL-C was significantly lower than the Han,in the diabetic group, no significant differences in the target.,Diabetic patients with coronary heart disease is lower than the mean age group of Uygur Han,BMI is higher than the Han.Uygurs in the control group BMI,,FBG,TG,APOB/A is higher than the Han.5) age,BMI,TG,LDL-C, LP-a is an independent risk factor for coronary heart disease,women are protective factors,LDL-C is one of the most prominent risk factors.Age,BMI,TC,LDL-C,LP-a coronary heart disease and diabetes are independent risk factors,women,HDL-C are protective factors.Age,TC,TG are an independent risk factor for diabetes.Conclusion: APOA5 allele C may be associated with increased TG and LP-a related,C allele are probably Uygur diabetes susceptibility gene with coronary heart disease;APOB gene A allele EocRI gene target with no obvious relationship between blood lipid,Uygur A allele and diabetic patients with coronary heart disease and coronary heart disease may be related;A alleles are possible coronary heart disease and diabetic with coronary heart disease susceptibility gene Uygur;CETP gene D442G in G and serum lipids and diabetes mellitus with coronary heart disease and coronary heart disease,diabetes probably does not matter.High blood cholesterol,high blood pressure,high blood sugar,body mass index increase in diabetes mellitus are risk factors for coronary heart disease and risk factors for coronary heart disease,hypertension,hyperlipidemia,increased body mass index are risk factors for diabetes.Uygur Han has more obvious than the lipid metabolism disorders,diabetes mellitus with coronary heart disease in younger age at onset Uygur.