Study On Therapeutic Material Basis Of Huodan Pills

Studying on the substance foundation of Chinese Herb Pharmacodynamic is the basis of research and development a new generation Chinese Patent Medicine with definite effective components,exact efficacy,stable and controllable quality,also the key to achieve modernization of Chinese traditional medicine.To investigate the substance foundation of Huodan pill by means of the Purification & Recombinant is the innovation in this project. According to the chemical composition and the feature in this formula,we could apply modern industrial technology to get various chemical compositions of Herba Pogostemonis' leaf and Porcine Gall Powder,and verify the active components by tracing and analysis the anti-inflammatory and anti-allergic chemical compositions with reference to traditional pill, which were used as compounds to Investigation on compatibility and dose optimization.It can lay a foundation of previous research for developing a new traditional Chinese medicine.Huodan pill,rooted from Yi Zong Jin Jian in Qing dynasty,is a famous Chinese Patent Medicine treating chronic rhinitis and chronic sinusitis.With the efficacy of resolving turbidity with aroma,clearing heat and relieving stuffiness,it can be used to treating intrinsic damp turbidity,nasal obstruction,nasal discharge and prefrontal ache accused by stagnant fire in Dan channel.It has a clinical application history more than two hundred years.Huodan pill is consist of two parts:Herba Pogostemonis' leaf and Porcine Gall Powder.Although the definite therapeutic effect,there are a large of disadvantages such as the rough product,old production process,backward dosage forms.The project developed a series of research as below:1 Screening of active part ofHerba Pogostemonis' leaf in Huodan pill. We screened active part of Herba Pogostemonis' leaf by pharmacodynamic test used in anti-inflammation and anti-hypersensitiveness drug,and used patent medicine Huodan pill as the control group.The results demonstrated that hydrophilic and lipophilic part without volatile oil of Herba Pogostemonis' leaf have the effect of anti-inflammation,but lower than volatile oil,total extraction of Herba Pogostemonis' leaf and the control group.At the same time,both parts have little effect of anti-hypersensitiveness.While volatile oil of Herba Pogostemonis' leaf have the same anti-inflammation effect as total extraction of Herba Pogostemonis' leaf and the control group,as well as the anti-hypersensitiveness effect.We concluded that volatile oil is the main active part of Herba Pogostemonis' leaf in Huodan pill.We extracted volatile oil from Herba Pogostemonis' leaf by two methods:SFE-CO2 and distillation,and the results showed that only aromatic water can be gotten by the method of distillation.So the SFE-CO₂ is adopted to extract volatile oil.The protocol of extraction is the following:Herba Pogostemonis' leaf is crashed to coarse powder first,and then extracted by SFE-CO₂.The extract temperature is 53℃,extract pressure is 13MPa,while the resolve pressure is 5.5Mpa,the resolve temperature is 63℃,the flow rate of CO₂ is 400L/h.The whole process lasted 2 hours.And then the volatile oil can be gotten. Volatile oil extracted by SFE-CO₂ accord to Herba Pogostemonis' oil in market,and both are consistent with Chinese Pharmacopeia.2 Evaluation ofHerba Pogostemonis' oil in Huodan pill. Refined HuoDan Recipe is comprised ofHerba Pogostemonis' oil and Porcine Gall Powder. We studied pharmacological effect of the refined HuoDan Recipe,Huodan pill,Herba Pogostemonis' oil,Porcine Gall Powder and results as showed below.a) Refined Huodan Recipe,Herba Pogostemonis' oil,Porcine Gall Powder and Huodan pill all have anti-inflammatory effects.The indexes include increasing capillary permeability of mouse belly cavity,swelling of rat toes by carrageenan, swelling of mouse auricle by dimethl benzene and rat cotton ball granuloma.,the acute and subacute inflammation model.Refined Huodan Recipe has the same inhibitory effect on the subacute inflammatory model such as swelling of rat toes by carrageenan,swelling of mouse auricle by dimethl benzene and rat cotton ball granuloma as Huodan pill,and better inhibitory effect on increasing capillary permeability of mouse belly cavity by acetic acid than Huodan pill.Herba Pogostemonis' oil and Porcine Gall Powder,synergize the anti-inflammatory effect, which implies that refined Huodan recipe has good anti-inflammatory effect as well as compositive materials,and which have synergism.b) There are all anti-allergy effect in Refined Huodan Recipe,Herba Pogostemonis'oil,Porcine Gall Powder and original prescription of Huodan pill. The indexes include allergia contractile response of ileum muscle in cobaya by egg albumin,passive cutaneous ana-phylaxis reaction(PCA),delayed hypersensitivity of mouse's pinna skin by 2,4-dinitro-chlorobenzene.Refined Huodan Recipe has higher depressant effect on Allergic Contractile response of cony pig by Ovalbumin and PCA of same species of rat than Huodan pill.The results have indicated that Refined Huodan Recipe and its disassembled prescriptions have remarkable anti-allergy effects.Drug synergy has the relationship of synergistic action.c) Refined Huodan Recipe has significant anti-microbial doughty effects.Refined Huodan Recipe has obvious dispatity degree anti-bacterial action against staphylococcus aureus,alpha hemolytic streptococcus,beta-hemolytic streptococci, streptococcus pneumoniae,micrococcus catarrhalis,corynebacterium diphtheriae, escherichia,aeruginosus bacillus,blastomyces albicans;The in corpore anti-microbial tests showed that Refined Huodan recipe has doughty inhibit activity to diplococcus lanceolatus,staphylococcus aureus,beta-hem olytic streptococci.These research results indicate that Refined Huodan recipe has anti-microbial doughty effects.d) Refined Huodan Recipe,Herba Pogostemonis'oil,Porcine Gall Powder and Huodan pill have significant enhancing immunity function effects.Drug synergy has the relationship of synergistic action.Refined Huodan Recipe,Herba Pogostemonis'oil,Porcine Gall Powder can obvious strengthen the phagocytosis of mice's reticuloendothelial system.Refined Huodan Recipe's enhancing immunity function effect is as the same as Huodan pill.The results have indicated that Refined Huodan Recipe and its disassembled prescriptions have remarkable enhancing immunity function effects.The results of the experiment indicated that Refined Huodan Recipe,which made of Herba Pogostemonis'oil and Porcine Gall Powder,havs notable anti-inflammatory effects, anti-allergy effects,anti-microbial effects and enhancing immunity function effects.Drug synergy has the relationship of synergistic action.The results show that substitute Herba Pogostemonis'oil for Herba Pogostemonis' leaf,Refined Huodan Recipe can reserve its effect. 3 Effect of Porcine Gall Powder composition for liver function and blood biochemical. Because of Porcine Gall Powder,the Huodan Pills have the disadvantage of difficult forming,the older preparation,large taken dose,foul smells and difficult controlling of the microorganism.In addition,Because of the amount of cholesterol and Chenodeoxycholic acid in Porcine Gall Powder,you will have cell toxicity,abnormal determination of cholesterol in your blood and liver function damage,if you take in a long time of Huodan pills.In order to explore a kind of preparation that has the advantage of defined effective medium,stable quality,well-defined Pharmacological effect,well-defined therapy and normal determination of cholesterol in your blood,although you take in a long time of them. It is very necessary to look for the active site or the effective component of the Porcine Gall Powder,which can Substitute of Porcine Gall Powder Using in Huodan Pills.The Cholic acid has many kinds of Pharmacological effect,especially in anti-inflammatory,anti-allergy, anti-microbial and enhancing immunity function effects.The other Cholic acid is very little, no notion of Pharmacological effect and some are hurt to liver function and cardiovascular system.Hyodeoxycholic acid is the main active component of Porcine Gall Powder,which has testified by modern research.It has the advantage of one chemical compound,stable quality, clearing Pharmacological effect,no foreign matter and no the amount of cholesterol and easy forming,which offers the theoretical support to Hyodeoxycholic acid substitute of Porcine Gall Powder using in Houdan pills.Porcine Gall Powder recipe can increase the level of cholesterol in blood and reduce the damage of the liver function,but Hyodeoxycholic acid recipe can not,if you take in a long time of them.The levels of cholesterol,AST,ATP in blood of the Porcine Gall Powder recipe low,middle,high dose groups is higher than the blank group,the level of ALT as the same as the blank group,after giving drug for 90 days.The levels of cholesterol,AST,ATP in blood of the Porcine Gall Powder recipe low,middle,high dose groups come back into normal level after stopping using drug for 14 days.The levels of cholesterol,AST,ATP,ALT in blood of the Hyodeoxycholic acid recipe low,middle,high dose group is the same as the blank group,after giving drug for 90 days and stopping using drug for 14 days. The results of the experiment indicate that using Hyodeoxycholic acid Substituting of Porcine Gall Powder Using in Huodan Pills makes to raise the safety of Huodan Pills. 4 Effect of Hyodeoxycholic acid for the pharmacodynamics of Huodan pill This effective compound recipe consists of Herba Pogostemonis'oil and Hyodeoxycholic acid could avoid from injuring the liver,but it can be still effective as Huodan pill? Four kinds evaluating indicator of rhinitis such as anti-inflammatory,anti-allergy,anti-bacterial and enhancing immunity function experiment were used to compare the pharmacology of the Hyodeoxycholic acid instuting ofPulvis Fellis Suis before and after.The conclusion is:a) Huodan effective component recipe,Hyodeoxycholic acid,Herba Pogostemonis'oil, Refined Huodan Recipe,Porcine Gall Powder and Huodan pill has significant anti-inflammatory effects.Hyodeoxycholic acid and Oleum pogostemonis have the relationship of synergistic action.Hyodeoxycholic acid recipe has the same inhibitory action on mice's auricle swelling induced by xylene,the increase of capillary permeability in mice induced by acetic acid,rat's toes swelling induced by carrageenan, the granuloma by the cotton ball as Huodan pills and Porcine Gall Powder recipe. Hyodeoxycholic acid has the same inhibitory action on mice's auricle swelling induced by xylene,the increase of capillary permeability in mice induced by acetic acid,rat's toes swelling induced by carrageenan,the granuloma by the cotton ball as Porcine Gall Powder.Hyodeoxycholic acid and Oleum pogostemonis have significant anti-inflammatory effects.Drug synergy has the relationship of synergistic action.The results of the experiment indicate that Hyodeoxycholic acid is the active component of the Porcine Gall Powder in anti-inflammatory pharmacological effects and Hyodeoxycholic acid may be institute of Porcine Gall Powder using in Huodan Pills.b) Huodan effective component recipe,Hyodeoxycholic acid,Herba Pogostemonis'oil, Refined Huodan Recipe,Porcine Gall Powder and Huodan pill has the relationship of synergistic action.Hyodeoxycholic acid,Oleum pogostemonis and Huodan recipe have obvious inhibitory action on extracorporeal ileum muscles' hypersensitive contractility reaction of Guinea pig sensitized by albumin,homogeneous passive cutaneous anaphylaxis in mice,mice's auricle cutaneous forme tardive supersensitivity action by 2,4-dinitrochlorobenzene.The results have indicated that Huodan recipe and its disassembled prescriptions have remarkable anti-allergy effects.Drug synergy has the relationship of synergistic action.c) Huodan effective component recipe,Hyodeoxycholic acid,Herba Pogostemonis'oil, Refined Huodan Recipe,Porcine Gall Powder and Huodan pill have significant enhancing immunity function effects.Drug synergy has the relationship of synergistic action.Hyodeoxycholic acid,Oleum pogostemonis and Huodan recipe can obvious strengthen the phagocytosis of mice's reticuloendothelial system.The results have indicated that Huodan recipe and its disassembled prescriptions have remarkable enhancing immunity function effects.Drug synergy has the relationship of synergistic action.d) Huodan effective component recipe,Hyodeoxycholic acid,Herba Pogostemonis'oil, Refined Huodan Recipe,Porcine Gall Powder and Huodan pill has significant anti-microbial doughty effects.Huodan recipe has obvious dispatity degree anti-bacterial action against staphylococcus aureus,alpha hemolytic streptococcus, beta-hemolytic streptococci,streptococcus pneumoniae,micrococcus catarrhalis, corynebacterium diphtheriae,escherichia,aeruginosus bacillus,blastomyces albicans; The in corpore anti-microbial tests showed that refined huodan recipe has doughty inhibit activity to diplococcus lanceolatus,staphylococcus aureus,beta-hem olytic streptococci.Huodan recipe has anti-microbial doughty effects.By studying on the effective fractions or components of Herba Pogostemonis' leaf and Porcine Gall Powder respectively,and comparing with the original formula,it could affirm that the effective parts are patchouli oil and hyodeoxycholic acid which have the synergistic interaction.Formulating with the effective compositions of Huodan pill not only could avoid the adverse reaction of the original prescription,but also overcoe the technical problems fundamentally such as large dosage,hard shaping and unstable quality.It offered a technological support for the high quality product of "safe,effective,stable,and controllable".This project has made clear the material basis of treating rhinitis.The formula consists of patchouli oil and hyodeoxycholic acid is a rudiment of the modern Chinese patent medicine. It provided scientific basis and laid a theoretical foundation for product renewal.This study method could offer a new research approach to illustrate the material basis of herbal compound.