Application Of Phage Display In Transdermal Drug Delivery And Bio-nanomaterials

Phage display is a mature technology for scanning protein interaction.We apply it into transdermal enhancer and nano-material binding.Efficient transdermal drug delivery of large hydrophilic drugs is challenging.Here we report that the short synthetic peptide,ACSSSPSKHCG,identified by in vivo phage display,facilitated efficient transdermal protein drug delivery through intact skin. Coadministration of the peptide and insulin to the abdominal skin of diabetic rats resulted in elevated systemic levels of insulin and suppressed serum glucose levels for at least 11 h.Significant systemic bioavailability of human growth hormone was also achieved when topically coadministered with the peptide.The transdermal-enhancing activity of the peptide was sequence specific and dose dependent,did not involve direct interaction with insulin and enabled penetration of insulin into hair follicles beyond a depth of 600μm.Timelapse studies suggested that the peptide creates a transient opening in the skin barrier to enable macromolecular drugs to reach systemic circulation.The calcitonin could reach blood circulation through skin with TD-1 at low dose and without TD-1 at high dose.The scale of nano-materials induces lots of biological effects.The modified nano-C60 will have more functions.The peptide scanned with phage display provides a foundation for modification.