Fat Source Of Flk-1 ~ + Mesenchymal Stem Cell Differentiation Process Microrna Expression

Adult mesenchymal stem cells are a group of cells in adult tissue with the capacity of self-renewal and multi-lineage differentiation. A lot of reports have been published in recent years regarding the differentiation potential of mesenchymal stem cell to mesodermal as well as ectodermal and endodermal cells. However, the detailed regulatory mechanism underlying these processes remains unclear. In this research, adult mesenchymal stem cells were isolated from human adipose tissue and induced to differentiate into glial cells, endothelial cells and epithelial cells. Specific microRNAs were selected based on previous microarray results and their expression profiles during above processes were dectected. To further explore the possible mechanism of miR-X's function in glial cell differentiation, its molecular targets were predicted through bioinformatic approach. Our data showed that miR-X and miR-Y had distinct expression profile in different lineage differentiation process, indicating that they may have important regulatory functions through post-transcriptional repression of functional target molecules.