Intraocular Transplantation Of Human Adipose-Derived Stem Cells In Rabbit With Experimental Retina Hole Model

Purpose:To investigate the morphological and differential changes of retinal hole repair tissue after human adipose-derived stem cells(ADSCs) transplantation in rabbit retinal hole models.And to investigate the differentiation of ADSCs in retinal hole eyes.Methods:20 New Zealand white rabbit were used.Pars plana vitrectomy was performed in the left eye.And a 1.5mm-diameter retinal hole was made with an extrusion needle.In one group of rabbits We injected 20μl/10μl human ADSCs suspension solution into the eyes at the surface of retinal holes. In another group phosphate buffered saline(PBS) was injected as control. To assess the changes of retinal holes,at 2,4,8,12,20,32 days postoperatively eyes were examined with a 3D-OCT.2D-OCT,3D-OCT and fundus images were obtained.At 12 and 32 days after surgery,eyes were enucleated and prepared in paraffin sections for observation by light microscopy and immunofluorescence detect with antibodies to human nuclei,glial fibrillary acidic protein(GFAP),Opsin and Protein kinase C(PKC)Results:3D-OCT showed that repairs occurred at the 4^th day after operation in both group.In the control group repair tissue proliferated as time passed by,and the maximum was reached at 20 days postoperatively.During the observation period,repair tissue was still thinner than the normal retina.And immunofluorescences showed that the area of retinal defect was covered with cells which were positive for GFAP only.In the ADSCs transplantation group the repair tissue grew more rapidly and generously, making a small bulge at the retina defect area.A proliferation membrane was formed In a 20μl transplantation group eye.Immuno-fluorescences showed that the area of retina hole was covered with cells which were positive for GFAP or Opsin or PKC.A few cells were positive for anti-human nucleus antibody(HuNu) in the recipient eyes,and also positive for GFAP or Opsin or PKC.Conclusions:These findings suggest that in this model retina holes repair and close spontaneously.Glial cell is the main cell type in the repair process.Retina hole repair process is promoted by Human ADSCs transplantation. The human ADSCs also stimulate differentiation of repair cells, which express markers of sensory retina cell or bipolar cell.And human ADSCs in the micro-environment of retinal injury obtain the ability of differentiation to retina cell types.