Inhibitory Effect Of Matrine On Androgen Dependent Prostate Cancer Cell Line LNCaP In Vitro

Purposc:To study the effect of matrine on the apoptosis and expression of AR and PSA inprostate cancer cells LNCaP.Methods:After being treated with 0.5～3.0g/L matrine for 12～36 h,the growth activities ofcancer cells were studied by MTT colorimetry and trypan blue stain assay.Cell cycle phases andapoptosis were inspected by flowcy-tometery(FCM).The levels of Bcl-2 and Bax were tested byWestern Botting.The Levels of AR were tested by Western Botting.The expression of prostatespecific antigen(PSA) was measured by AXSYM system-chemical luciferase methods.Results:Matrine could effectively inhibit the in vitro growth of androgen dependentprostate cancer cell line LNCaP in time-and-dose-dependent manners(P＜0.01).Cell cycleswere arrested at G₂/M phase(P＜0.01).Partial cancer cells presented apoptosis(P＜0.05).Matrine could decrease in the levels of Bcl-2 and increase in the levels of Bax in cell lineLNCaP in a dose-dependent manner(P＜0.01).Matrine could decrease in the levels of ARin cell line LNCaP in a dose-dependent manner(P＜0.01).Matrine inhibited the expressionof PSA on prostate cancer cells(P＜0.05).Conclusions:Matrine could suppress the growth of LNCaP,and promotes their apoptosis.Matrine also inhibited the expression of AR and PSA on prostate cancer cells.Key words:prostate cancer;matrine;LNCaP cells;androgen receptor;prostate specific antigen...