The Immune Response Of Mouse Upper Respiratory Tract Vaccinated By FMDV Epitopes Peptide With Molecular Adjuvants

Upper respiratory tract immunity as a novel way of immunization, more workers in immunology research got into it. A lot of infectious disease infected body from the respiratory tract, which is the best research strategy to research how to control the virus provides. Mucosal adjuvant as an important component of mucosal immunity, a lot of scholars are researching immune enhancer for mucosal immune, but the mechanism of majority of adjuvants are unknown. FMDV as an enormous economic and political impact of the disease, has brought hundreds of millions of human beings in economic losses, Investigating the prevention and controling of the popular new subtype of large high-throughput vaccine is imminent.In this study, the upper respiratory tract mucosal immunity, FMDV peptide epitopes, CTB adjuvant, gene adjuvant and cytokine adjuvants were studied in mice. Molecular biology techniques, genetic engineering technology, bio-informatics technology and methods of reverse engineering DNA technology were used to build the Asia 1 FMDV peptide epitopes with CTB adjuvant by Linker Protein, we got a peptide 31C. Reverse transcription from the adult cow total RNA,we cloned BoIFN-γgene and purified mature BoIFN-γprotein peptide 31C as a adjuvant polypeptide. Comparing the peptide and the virus group, cytokine gene adjuvant and peptide adjuvant group, upper respiratory tract immunity and subcutaneous injection immunization group, CTB adjuvant group, we got the level of sIgA and serum antibody levels of humoral immunity by indirect ELISA detection, the level of cell-mediated immunity by lymphocyte proliferation assay, detectde the Change of IL-2, IL-4, IL-10 and IFN-γin the neck lymphatic, lung and spleen by Realtime RT-PCR. Analysing the data obtained through the above-mentioned we got the changes of protection of mucosal immunity, humoral immunity of the whole body, systemic cellular immunity and cytokines in various organs of the regulation changes.Experimental data show that the immunological activity of 31C was significantly higher than the commercialization of peptide epitopes, 31C polypeptide could make the mice get sIga and humoral antibody. Nasal immunization induced the reproductive tract get sIgA. Nasal immunization got memory cells more earlier than the other group. The CTB adjuvant can help significantly enhance epitope peptide immunogenicity. CTB not only enhance the level of sIgA by nasal immunization, It could significant increase the activity of the epitope protein immunogenicity on it. The mBoIFN-γcan significantly enhance IFN-γexpression and sIgA levels in the injection site, and to enhance the cellular immunity level of body . The pBoIFN-γgene injected in the body as a mucosal adjuvant had The immune-enhancing effects for a long time, at the inject sites, got the tolerance to second vaccination.Researching about the mucosal immunity and immune peptides stiff job, this experiment got a lot of breakthrough in it.