Pre-Moxibustion Prevents Acute Damnification Of Gastric Mucosa From Apoptosis And Mitochondria-Mediated Pathway Through Inducing High Expression Of Heat Shock Protien 70

Objective:To observe the effects of pre-moxibustion on the expression of heat shock protein 70(HSP70) in gastric mocosa of acute damnification of gastric rats.To observe the effects about HSP70 of moxibustion on apoptosis mitochondrial pathway and the content of Cytochrome C(Cyt-c),apoptotic protease-activating factor-1(Apaf-1),cysteine aspartic acic specific protease-9(Caspase -9),Caspase-3.To explore the relationship between the apoptosis mitochondrial pathway and HSP70,and the mechanism of pre-moxibustion at the acupoints of Foot-Yangming meridian in preventing gastric mucosa systemly.Methods:There are three sections of this problem.The first section:Thirty-two healthy Wister rats were randomly assigned to blank,binding,moxibustion at "Zusanli" and "Liangmen" and moxibustion at control points groups.Assaying the content of HSP70 in heart,brain,gastric mucosa by immunohistochemical method and observing the express HSP70mRNA in gastric mucosa by Real-Time PCR method.The second section:Forty healthy Wister rats were randomly assigned to binding,model,moxibustion+model,quercetin+moxibustion+model,quercetin +model groups.Ethanol lavage in stomach method was adopted for acute damnification of gastric mucosa rat model.After quercetin blocking HSP70 synthesis,we observed the expression of ulser index(UI) by Guth method, apoptosim index(AI) by TUNEL method,and HSP70 by western-blot method.The third section:Thirty-two healthy Wister rats were randomly assigned to binding,model,moxibustion point+model,moxibustion control point+model groups.Assaying the content of Cyt-c by western-blot method and observing HSP70,AI,Apaf-1,Caspase-9,Caspase-3 by immunohistochemical method in gastric mucosa.Results:After treated with ethanol,the UI and AI increased,the expression of HSP70 in gastric mucosa decreased(P＜0.05 or P＜0.01).At the same time, the content of Cyt-c,Apaf-1,Caspase-9,Caspase-3 increased highly(P＜0.05 or P＜0.01) compared with binding group.While treated with pre-moxibustion at"Zusanli" "Liangmen",the UI and AI in gastric mucosa significantly decreased,the expression of HSP70 in gastric mucosa increased clearly(P＜0.05 or P＜0.01).At the same time,the content of Cyt-c,Apaf-1,Caspase-9,Caspase-3 decreased highly(P＜0.05 or P＜0.01) compared with model and moxibustion control point+model group.Conclusion:Moxibustion at "Zusanli" "Liangmen" had the protective effects on gastric mucosa of acute damnification in rat.And the mechanism of inhibition apoptosis may be promoting the synthesis HSP70,decreasing the content of Cyt-c,Apaf-1,Caspase-9,Caspase-3 so as to suppressing the cell apoptosis mitochondrial pathway.