| Objective:To study the relationship between abortion and the expression of mouse SOCS1,2,3 and Th1/Th2 type cytokines at the maternal-fetal interface. To evaluate the role that Shoutaiwan regulate SOCS1, SOCS3mRNA expression and Thl/Th2 type cytokine expression and balance of control in maternal-fetal interface and By cultured in vitro,to study shoutaiwan can regulate gene expression of different SOCS and the impact of Thl/Th2 immune deviation and the adjustment mechanism. In order to shoutaiwn can induce the maternal-fetal immune tolerance Of mechanism.Methods:1.The abortion-prone CBA/JxDBA/2 matings were established as the model of spontaneous abortion and the nonabortion-prone CBAxBALB/c matings were used as the model of normal pregnancy.The model of spontaneous abortion CBA/JxDBA/2 of mice pregnant were randomly divided into four groups according to the sequence of pregnancy, From the 1st day of pregnant,pregnant rats of every group are oral administration in different doses (Og/kg,3g/kg,6g/kg, 12 mg/kg).on the 14th days of pregnancy,the mice are killed. The embryo resorption rate was counted,RT-PCR was applied to analyze the SOCS1, SOCS2,SOCS3 transcription of decidua and placenta, ELISA Was applied to determinate the IL-4, IL-10, IFN-y, TNF-a concentration in culture supemates of decidual and placenta, The ratio of IL-4/IFN-y, IL-10/TNF-a was used as the index to evaluate the immune regulation balance.2.the CD4+T cells coming from the non-pregnant BALB/c female spleen cells are think as respond cell, the inactivation of DBA/2 male spleen cells are think as APC t, add OVA antigen, IL-2 and IL-12 Co-culture of cytokines, induce CD4+T cells Thl diviated model. shoutaiwan serum containing low middle high-dose and the most effective dose of Oh, 1h,2h,4h,8h, respectively, induce CD4+T cells of Thl deviation differentiation in vitro model, RT-PCR was applied to analyze the SOCS1, SOCS2,SOCS3 transcription of decidua and placenta, ELISA Was applied to determinate the IL-4, IL-10, IFN-y, TNF-a concentration in culture supemates of decidual and placenta, The ratio of IL-4/IFN-γ, IL-10/TNF-a was used as the index to evaluate the immune regulation balance.Results:The models of embryo resorption rate are higher than the normal pregnancy group, Compared with model group, low-dose shoutaiwan can not significantly improve the model of spontaneous abortion in pregnant mice embryo resorption rate (P> 0.05); shoutaiwan high dose group of pregnant rats absorption rate of the embryos were significantly decreased, IFN-y, TNF-a expression in model group are significantly higher than the normal pregnancy group (P<0.05), IL-4, IL-10 expression, IL-4/IFN-y, IL-10/TNF-a ratio are significantly lower than the normal pregnancy group at Maternal-fetal interface (P<0.05), Compared with model group, shoutaiwan low middle high-dose group can decreased significantly TNF-a and IFN-y expression of maternal-fetal interface of pregnant rats (P<0.05), shoutaiwan middle high dose group make IL-4, IL-10 expression in maternal-fetal interface increased significantly (P<0.05). Compared With the normal pregnancy group,the expression of IL-4, IL-10, IFN-y, TNF-a of maternal-fetal interface in pregnant rats show no significant change (P> 0.05); shoutaiwan low middle high-dose group shift Thl/Th2 balance towards Th2, the middle high-dose group show no significant difference,compared with the normal group (P> 0.05).when compared with normal pregnancy,no significant difference between groups (P> 0.05).SOCS1, SOCS2, SOCS3mRNA in the model group and normal pregnancy decidual and placental tissue were expressed, compared with the normal pregnancy group, SOCS1mRNA increase in the model group, SOCS3mRNA expression and, SOCS2mRNA show no significant differences (P> 0.05). shoutaiwan low middle high dose group model can reduce SOCS1 gene expression of maternal-fetal interface of pregnant rats, shoutaiwan middle high dose group increase model group SOCS3 gene expression (P<0.05). shoutaiwan low middle high dose group in pregnancy was no significant difference from the normal expression of SOCS1, shoutaiwan middle hign (?)ose group of SOCS3 gene expression are no significant difference in normal pregnancy with (P> 0.05). The models of embryo resorption rate are higher than the normal pregnancy group, SOCS1, SOCS2, SOCS3mRNA in the model group and normal pregnancy decidual and placental tissue were expressed, compared with the normal pregnancy group, SOCS1mRNA increase in the model group, SOCS3mRNA expression and, SOCS2mRNA show no significant differences (P> 0.05). Maternal-fetal interface IFN-y, TNF-a expression in model group are significantly higher than the normal pregnancy group (P<0.05), IL-4, IL-10 expression, IL-4/IFN-y, IL-10/TNF-a ratio are significantly lower than the normal pregnancy group (P<0.05). Middle and high dose Shoutaiwan containing serum could significantly decrease the expression of SOCS1, IFN-y, TNF-a(P<0.05).High dose Shoutaiwan-containing serum could significantly increase the expression of SOCS3, IL-4, IL-10 (P<0.05).In addition,high dose Shoutaiwan-containing serum could significantly decrease the expression of SOCS1, IFN-y, TNF-arespectivly at 2h,4h and 8h after treating (P<0.05),the peak was 4h.High dose Shoutaiwan-containing serum also significantly increase the expression of SOCS3,IL-4, IL-10 respectivly at 2h and 4h (P＜0.05),the peak was 4h.Conclusion:The maternal-fetal interface in normal pregnancy group showed a significant local Th2-type immune bias. And the model group showed a significant bias toward Thl-type immunity. Maternal-fetal interface Th1/Th2 type cytokine imbalance are an important cause one of the reasons for abortion. SOCS1, SOCS3 play an important role in regulate the balance of Thl/Th2 type. shoutaiwan can reduce the expression of SOCS1 and SOCS3 at maternal-fetal interface,and recover maternal fetal interface Thl-type/Th2-type cytokine expression of the physiological balance, induce the immune tolerance to the maternal-fetal, thereby reduce the rate of embryo resorption.
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