Study Of The Expression Of SDF-1 And MMP-7 In Human Breast Cancer And The Relationship Between Them

Part one:Expression of SDF-1,MMP-7 and Alpha-SMA in breast cancerObjective:To Study expression of Stromal cell-derived factor-1(SDF-1),MMP-7 and Alpha-Smooth Muscle Actin(Alpha-SMA) in breast cancer by Immunohistochemical.Methods:To observe the expression of SDF-1,MMP-7 and Alpha-sma in breast cancer tissue,cancer nearby tissue and normal breast tissue,and contrast the expression of SDF-1 and Alpha-SMA in vicinity paraffin section.Results:The expression of SDF-1,MMP-7 and Alpha-SMA were higher in breast cancer tissue and cancer nearby tissue;there are similar position and intensity expression of between SDF-1 and Alpha-SMA. And there was a relationship between the expression of SDF-1 and MMP-7.Conclusion:1.SDF-1,MMP-7 and alpha-SMA were high expression in breast cancer tissue and cancer nearby tissue,they were all correlated with tumor.2.The expression of SDF-1 was correlation with the expression of MMP-7. Part two:Study the growth and invasion influence of degrdn extracellular matrix byâ…£collagenase in humam breast cancer line MCF-7Objective:To observe the growth and invasion influence of degrdn extracellular matrix byâ…£collagenase from breast cancer stroma on humam breast cancer line MCF-7.To study the activity of DECM andâ…£collagenase in the tumor degradation and the relationship between SDF-1 and MMP-7 protein influenced by the DECM.Methods:Segregating stroma and cell through digestion by pamcreatin from normal breast tissue,cancer side tissue and cancer tissue,then extracted stroma,observingâ…£collagenase degradate stroma in different groups,there were 7 groups:1.blank group,2.normal breast tissue group(Noâ…£collagenase),3.normal breast tissue group(â…£collagenase),4.brast cancer side group(Noâ…£collagenase),5.brast cancer side group(â…£collagenase),6.breast cancer group(Noâ…£collagenase),7 breast cancer group(â…£collagenase),the same quantity DECM were added in human breast cancer line MCF-7.The tumor cell growth was observed by soft agar clone formation method,the tumor cell metastasis was studed by Transwells method,the expressive of MMP-7 and SDF-1 protein were detected in MCF-7 by Western blot method.Results:The DECM from breast cancer stroma could promote human breast cancer line MCF-7 proliferate and invasive,there was significantly distinction betweenâ…£collagenase group and No collagenase group in the same tissue,the expression of SDF-1 and MMP-7 were elevated by DECM.Conclusion:1 The DECM fromâ…£collagenase degrdn breast cancer stroma possessed tumor biologic activity which promoted hu man breast cancer line MCF-7 proliferate and invasive.2 Degrdnin g stroma in tumor could simultaneously elevate the expression of SDF-1 and MMP-7. Part three:The interaction of SDF-1 and MMP-7 in humam breast cancer line MCF-7Objective:To Study the biological influence of SDF-1 in MCF-7 and the interaction of SDF-1 and MMP-7.Methods:The tumor cell proliferation and invasion were observed in MCF-7 by different concentration SDF-1,the expression of MMP-7 was detected by Western blotting with different density SDF-1 and at different time.In the same way,the expression of SDF-1 was detected with different density MMP-7 and at different time.The expression of MMP-7 was detected by Western blotting after using CXCR4 inhibitor T140 in MCF-7,the expression of SDF-1 was detected after using small interfere RNA anti-MMP-7 in MCF-7 by Western blotting.Results:SDF-1 enhanced tumor cell proliferation and invasion of MCF-7 in a concentration-dependent way.SDF-1 could upregulate the expression of MMP-7 in concentration- and time-dependent way.And vice versa.The expression of MMP-7 was down regulated after inhibited the expression of SDF-1 by T140,and SDF-1 also was down regulated after inhibited MMP-7 by siRNA.Conclusion:1.SDF-1 could enhance tumor cell proliferation and invasion in the breast cancer line MCF-7 in a concentration- and time-dependent way.2.There was interaction between SDF-1 and MMP-7,it seems like a regenerative feedback between them.