The Change Of Excitability Amino Acid, Glucose, Lactic Acid In Mouse Brain Extracellular Fluid After Injury And The Protective Function Of Gangliosides GM1

Objective: To study the change of excitability amino acid(EAA), glucose, lactic acid in mouse brain extracellular fluid(ECF), and to study the protective function of gangliosides GM1 on mouse brain.Methods: The change of Glutamic acid(Glu) in ECF of the control group A (sham-operation group) is not significant after injury(P> 0.05). In injury group B group (injury group), C group (treatment group), the basic level of Glu is same as that of control group A , Glu rised rapidly after injury, the undulation of Glu is obvious, and is highest during 15-30min after injury, Glu of group B and group C were 14.54±1.66 and 8.13±0.65μM respectively (P < 0.05), were higher than the control group obviously(P < 0.05), then reduced gradually to the level of group A after 75-90min.The change of lactic acid in ECF of the control group A (sham-operation group) is not significant after mjury(P> 0.05). In injury group B group (injury group), C group (treatment group), the basic level of lactic acid is same as that of control group A , lactic acid ascended rapidly after injury, the undulation of lactic acid is obvious, and is highest during 15-30min after injury, lactic acid of group B and group C were 0.83±0.07 and 0.75±0.06 u M respectively (P < 0.05), were higher than the control group obviously(P < 0.05), then reduced gradually to the level of group A after 75-90min .The change of glucose in ECF of the control group A (sham-operation group) is not significant after injury(P> 0.05). In injury group B group (injury group), C group (treatment group), the basic level of glucose is same as that of control group A , glucose descended rapidly after injury, the undulation of glucose is obvious, and is highest during 15-30min injury, glucose of group B and group C were 0.27±0.02 and 0.33±0.02mM respectively (P < 0.05), were higher than the control group obviously(P < 0.05), then ascended gradually to the level of group A after 75-90min.Conclusion:, In the dialyzate, excitability amino acid ascended continually in the early after injury, then to a peak value, this confirmed the relationship between the brain injury and excitability amino acid .Simultaneously, it was the theory basis for the clinical treatment, and identified the medicine time window.After the early use of GM1, peak value of the excitability amino acid droped obviously, hinted that GM1 have the explicit protective function on the brain injury which caused by the excitability amino acid. Provided the reference for the further clinical utilization.After the brain injury, the early use of GM1 reduced the stack of lactic acid in brain tissue , and increased the content of glucose, thus improved energy metabolism , and were good for neuron cell, which may afford useful clues for the treatment of brain injury with GM1...