The Clinical Experience Of Professor San Zhao Wei In The Treatment Of Postoperative Gastric Cancer And The Mechanism Of The Apoptosis Induced By Qi Zhu Formula In MGC-803

Objective:Gastric cancer is one of the highest fatality and malignant tumor in China.Clinically, the patients with surgical treatment of about 5%early gastric cancer,about 50%advanced gastric cancer died in 5 years due to relapse,even made a radical operation,there is still 30% -80%of local or distant recurrence.Therefore,it is the most pressing issues to be addressed that how to reduce or prevent tumor recurrence and metastasis,to improve the clinical efficacy and long-term survival in patients with advanced gastric cancer.Studies show that the incidence of gastric cancer is a multi-stage and multi-factor in the complex process of development.Tumor occurrence and development is not only a process of tumor cell proliferation and differentiation abnormalities,but also a process of anti-apoptosis in the changes in gene and protein,and the adjustment mechanism of the disorder will lead to the occurrence of tumor.Therefore,the induction of apoptosis in cancer becomes an important strategy in recent years.The role of Chinese medicine in the field of cancer therapy is increasingly important for the people.In order to further elucidate its mechanism,it is of view a great deal of research from the cell apoptosis to its associated protein,gene expression point.Large number of studies showed that many of the components of traditional Chinese medicine may block the tumor occurrence and development of a sound prevention and treatment of tumors,and it has a unique advantage on the effect of postoperative recurrence and metastasis of gastric cancer treatment.We draft for the rule of Yiqi Yangyin,Jieduhuayu Law and the QiZhu formulae by the characteristics of postoperative gastric cancer research.Therefore,we carried out both experimental and clinical studies to validate its efficacy and mechanism from both theoretical and clinical aspects after the treatment of gastric cancer.We explore the treatment of gastric cancer patients to learn from the methods and ideas for the therapy of tumor recurrence and metastasis.Methods:1.Clinical parts:we investigate the influence on the quality of life by the Quality of life questionnaire-Core30 and to sum up mentor Prevention of postoperative recurrence and metastasis of gastric cancer of academic thought.2.Experimental parts:In order to observe the effects of proliferation and apoptosis on Human Stomach Cancer Cell Line MCG-803 induced by Qi Zhu Formulae,we cultured the cells with routine methods and used the method of Flow cytometry and Western blot.Results: 1.The quality of survivals could be improved by Qi Zhu Formulae,and there is significant difference(p＜0.05).2.The growth of Human Stomach Cancer Cell Line MCG-803 could be effectively prohibited by Qi Zhu Formulae,and cell cycle was arrested on G1 phase.3.The expression of cytochrome c and changes of mitochondrial membrane potential could be induced by Qi Zhu Formula.Conclusions:1.The quality of survivals could be improved by Qi Zhu Formula,and it is an effective medicine that could prevent the recurrence of gastric cancer.2.Mitochondrial pathway plays an important role in the apoptosis on human stomach cancer cell line MCG-803 induced by Qi Zhu Formulae.