| Purpose:Effects of Xia xi Particles on blood pressure and extra cellular matrix of myocardial in spontaneously hypertensive rats.Methods:25 12-week-old male or female SHR were randomly divided into blank SHR group, valsartan group, the Xia Xi Particle high-dose group, middle dose group, low-dose groups of 10, and the other from 8 WKY rats as a normal control group, administration of 8 weeks to observe the following indicators.Results:1 Effects of Xia xi Particles on blood pressure and SHR cardiac function and structure1.1 behavior and general condition effect:Xia xi Particles can improve the behavior and general state of SHR, That indicates that Xia Xi Particles has regulation function.1.2 Decreasing blood effect: Xia xi Particles can inhibit the SHR blood pressure increased with age trends,4～6 weeks later compared with the blank group that there is significant difference (P <0.05), and antihypertensive effect of a smooth durable and effective.1.3 Reversing left ventricular hypertrophy(LVH) effect: Xia xi Particles reduced SHR left ventricular mass index (LVWI), compared with blank control group were significantly different (P <0.01), can reverse LVH to a degree. Morphological observation of the heart: 8 weeks after delivery, Xia xi particles can significantly reduce the SHR myocardial hypertrophy, reduction of extracellular matrix hyperplasia prompted and can reduce heart damage.2 Effects of Xia xi Particles on myocardial extracellular matrix2.1 PICP,PⅢNP,AngII effect: Studying the density of PICP,PⅢNP,AngII in serum of SHR with Enzyme-Linked Immunosorbnent Assay (ELISA) . The results show that, SHR serum PICP, PⅢNP, AngⅡconcentration was significantly higher than WKY control group (P <0.01); Given different doses of Xia xi Particles control PICP, PⅢNP, AngⅡlevels are also different,positive medicine group and the Xia xi Particles high dose group compared with the WKY group was no significant difference (P > 0.05),but the medium dose , low-dose group compared with the WKY control group, there is significant difference (P <0.05), in PICP low dose group compared with no significant difference in SHR, while the other two groups have differences. Reveals the I-type collagen, III collagen may be increased SHR ventricular hypertrophy, one of the material basis. Xia xi Particles can reduce the I-type collagen, III collagen and AngⅡconcentration exert on the extracellular matrix SHR opsonization played the role of improving the stromal hyperplasia.2.2 MMP-1 effect: Studying the density of MMP-1 in serum of SHR with Enzyme-Linked Immunosorbnent Assay (ELISA) . SHR serum MMP-1 concentration was significantly lower than WKY control group (P <0.01); Positive drug group and the Xia xi Particles high dose group compared with the WKY group was no significant difference (P > 0.05), but medium and low-dose group compared with the WKY control group, there is significant difference (P <0.05);2.3 The influence of Xia Xi Particles to myocardial TGF-β1 expression:By immunohistochemical method (ICH) Detect Myocardial myocardial expression of TGF-β1, by reversing transcription polymerase chain reaction (RT-PCR) detection of myocardial TGF-β1 gene expression levels. The results of Xia Xi Particles of SHR with reduced myocardial TGF-β1 content in the role, and reduce myocardial TGF-β1 protein and gene expression. Xia Xi Particles can be revealed by inhibiting transforming growth factor, exert systemic regulation of the function of the SHR to lower over-expression of extracellular matrix, with similar angiotensin receptor antagonist (ARB) of the lower blood pressure mechanism.Conclusion:一,The studies show that transfer of liver and kidney, Tongluo detoxification treatment and prescriptions (XiaXi particles) at different times of administration of hypertension, there is the exact role of slow rise in blood pressure and effect a smooth, durable, ease to the whole administration the role of the best groups in order to reveal some of the clinical group thought to the feasibility and reasonableness.二,XiaXi particles Can effectively improve myocardial hypertrophy and extracellular excessive collagen synthesis, suggesting that the drug can reverse the situation left ventricular hypertrophy, the heart must have a protective effect.三,XiaXi particles be able to effectively control serum PICP, PⅢNP, MMP-1 of the dynamic balance, and indirectly reflects the myocardial cells of the major components of extracellular matrix I, III collagen protein synthesis and degradation of the regulation. And show that serum PICP, PⅢNP is likely to be judged after clinical myocardial extracellular matrix to determine the extent of target hyperplasia.四,XiaXi particles can inhibit AngII, TGF-β1 protein over-expression, suggesting that the proliferation of extracellular matrix has the only fixed factor inhibition...
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