The Ionic Products Of Dicalcium Silicate Coatings Dissolution Enhances Bone Formation In Vitro

Partâ… Bioactivity of Plasma Sprayed Dicalcium Silicate Coatings In DMEM Culture FluidObjective To observe bioactivity of plasma sprayed dicalcium silicate coatings in DMEM culture fluids. Methods Dicalcium silicate coatings on titanium alloys substrates were prepared by plasma spraying and immersed in DMEM culture fluid for 7 day. SEM and EDS were used to observe surface morphologies and determine the composition of dicalcium silicate coatings before and after immersion in DMEM culture fluid. The elementary content of calcium (Ca), silicon (Si) and phosphorus (P) in this solution were determined by inductively coupled plasma (ICP) analysis. Results A dense carbonate-containing hydroxyapatite (CHA) layer was formed on the surface of the plasma sprayed dicalcium silicate coating soaked in DMEM solution for 7days. There were difference in Ca, Si and P ionic concentration(p<0.05) between the dicalcium silicate coating conditioned DMEM solution and control group culture fluid. Conclutions The results obtained indicated that the plasma sprayed dicalcium silicate coating possesses excellent bioactivity.Partâ…¡Observation of Ionic Products of Dicalcium Silicate Coating Dissolution to The Change of Osteoblast Morphology in Cell CultureObjective To observe the changes of osteoblast morphology during cell culture .In order to understand the effects of Dicalcium Silicate Coating dissolution on the growth of osteoblast. Methods Dicalcium silicate coatings on titanium alloys substrates were prepared by plasma spraying and immersed in DMEM culture fluid for 7 day. The elementary content of calcium (Ca), silicon (Si) and phosphorus (P) in this solution were determined by inductively coupled plasma (ICP) analysis. The full DMEM was prepared to be the control group culture fluid, the DMEM culture adding with ionic products of dicalcium silicate coating dissolution was prepared to be the experimental group culture fluid. The human osteoblast-like MG63 cells were cultured by experimental group culture fluid and control group culture fluid respectively to observed the morphological difference in general of osteoblast in the process of growth in culture and the difference in cell population (MTT chromatometry and staining Cytometry). Results There were difference in Ca, Si and P ionic concentration(p<0.05) between experimental group culture fluid and control group culture fluid. The cells of experimental group have better cell differentiation than the osteoblast of control groups. There was no difference in cell population (p>0.05) between the experimental group culture fluid and control group culture fluid. Conclutions The ionic products of dicalcium silicate coating dissolution can increase differentiation of human osteoblasts and can not increase cell population.Partâ…¢The Effect of Ionic Products of Dicalcium Silicate Coating Dissolution to Osteoblast Growth Cycle and ApoptosisObjective To observe the change of esteoblast growth cycle and apoptosis to understand the effect of ionic products of dicalcium silicate coating dissolution to osteoblast proliferation. Methods The full DMEM was prepared to be the control group culture fluid, the DMEM culture adding with ionic products of dicalcium silicate coating dissolution was prepared to be the experimental group culture fluid. The human osteoblast-like MG63 cells were cultured by experimental group culture fluid and control group culture fluid respectively. The osteoblast growth cycle and apoptosis was determined by flow cytometry at 3,6 and 12 day. The cell proliferative activity indicatrix[SPF(S-phase fraction) and PI(proliferous index) ] and apoptotic index(AI) were compared. Results On days 3,there were no difference in the percentage of cells in the G1 phase and PI (p>0.05) between the experimental group and control group . there was a significantly lower percentage of cells in the S phase on days 3 (P<0.05) in experimental group. In 6 to 12 day, there were significantly higher percentage of cells in the S phase and PI (P<0.01),and wae significantly lower percentage of cells in the G1 phase in experimental group. Apoptosis was markedly increased in the experimental group at days 3 and 12 (P< 0.05; P<0.01). Conclusion The ionic products of dicalcium silicate coating dissolution can increase proliferation of human osteoblasts by increased cell cycling. The feature of the ionic products of dicalcium silicate coating dissolution increased proliferation of human osteoblasts is that it has slightly depressant effect on proliferation of human osteoblasts in the initial stage, but it can significantly increase proliferation of human osteoblasts in later stage. Increase in apoptosis possibly profits to osteoblast differentiation .Partâ…£The Effect of Ionic Products of Dicalcium Silicate Coating Dissolution to Osteoblast Differentiation and Phenotypic ExpressionObjective To further investigate the possible mechanisms of ionic products of dicalcium silicate coating dissolution which enhances bone formation by observed the effect of ionic products of dicalcium silicate coating dissolution to osteoblast differentiation and phenotypic expression . Methods The full DMEM was prepared to be the control group culture fluid, the DMEM culture adding with ionic products of dicalcium silicate coating dissolution was prepared to be the experimental group culture fluid. The human osteoblast-like MG63 cells were cultured by experimental group culture fluid and control group culture fluid respectively. Detected the express of collagen-â… ,bone gla-protein (BGP),osteoblast core-binding factor alpha-1(CBFA-â… )and insulin-like growth factorâ…¡(IGF-2) by polymerase chain reaction(PCR) and detected the activity of alkaline phosphatase(ALP) by biochemistry method. Results There were significantly higher express of collagen-â… ,BGP,CBFA-â… and IGF-2 (p<0.01) in experimental group in 3 to 12 days. Conclusion The ionic products of dicalcium silicate coating dissolution can increase proliferation , differentiation, formation of metrical and mineralization of human osteoblasts,and it profits to enhance bone formation.