Apolipoprotein A-I Inhibits Chemotaxis, Adhesion, Activation Of THP-1 Cells And Improves The HDL Inflammatory Index

Whereas the antiinflammatory effects of high density lipoprotein(HDL) are well described,such effects of Apolipoprotein A-I(ApoA-I) are less studied.On the basis of our previous study,we furtherly explored the mechanism of antiinflammatory effects of ApoA-I,and focused especially on the interaction between monocyte and endothelial cells and plasma HDL inflammatory index in LPS-challenged rabbits.The results showed that ApoA-I significantly decreased LPS-induced MCP-1 release of THP-1 cells and oxLDL-induced THP-1 migration ratio(P＜0.01 respectively). ApoA-I significantly decreased sL-selectin,slCAM-1 and sVCAM-1 release of LPS-stimulated THP-1 cells(P＜0.01,P＜0.01,P＜0.05 respectively).Furthermore, ApoA-I significantly inhibited LPS-induced CD11b and VCAM-1 expressions on THP-1 cells(P＜0.01,P＜0.05 respectively).ApoA-I diminished LPS-induced mCD14 expression(P＜0.01) and NFκB nuclear translocation in THP-1 cells.After single dosage treatment of ApoA-I,the value of plasma HDL inflammatory index in LPS-challenged rabbits was improved significantly(P＜0.05).Conclusions:ApoA-I can inhibit the chemotaxis,adhesion and activation of THP-1 cells and impove plasma HDL inflammatory index with presenting beneficial antiinflammatory effects.