Effect Of Shiquandabu Tang Granules On Hematopoietic Regulation In Myelosuppressed Mice

Objective:Myelosuppression is followed, when the cancer patients undergo radiotherapy and chemotherapy.The basic principle of treatment for myelosuppression is based on "Fuzheng" mainly. In this study, our aim was to explore the effect of Shiquandabu Tang Granules on hematopoietic regulation and its mechanisms, and provide experiment data for the improvement form of medication.Methods:In the present study, the model of myelosuppressed mice was prepared by 60Coγirradiation, cyclophosphamide injection and chloramphenicol injection. Hematopoietic progenitor cells culture, flow cytometry, immunohistochemistry(IHC), western blot method(WB), Enzyme-linked immuno-sorbent assay (ELISA), as well as semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) technologies are used to study the hematopoietic effects, proliferation of bone marrow nuclear cells, and cell cycle, cyclin, cyclin-dependent kinase, as well as bone marrow stromal cells, extracellular matrix of bone marrow hematopoietic microenvironment and other aspects. We explored systematically and deeply the effect of Shiquandabu Tang Granules on hematopoietic system and its mechanisms.Results:1 Within a certain dose range, Shiquandabu Tang Granules could significantly increase the number of white blood cell(WBC), red blood cell(RBC), hemoglobin(Hb) and platelet (PLT); increase the number of bone marrow nucleated cells; increase hematopoietic tissue area in bone marrow;2 Shiquandabu Tang Granules could effectively improve the colony forming-unit-erythroid(CFU-E),burst colony forming-unit-erythroid (BFU-E), colony forming-unit-granulocytemacrophage(CFU-GM), colony forming-unit-megakaryocyte(CFU-Meg);3 There has no significant difference between the Shiquandabu Tang Granules and the traditional decoction in improving the bone marrow hematopoietic function of myelosuppressed mice; 4 Shiquandabu Tang Granules could promote bone marrow cells into the cell cycle, promote the G0/G1 phase cells into S phase, S phase cells into G2/M phase transformation, thereby promoting the proliferation of bone marrow cells.5 Shiquandabu Tang Granules could promote gene expression of cell cycle regulatory protein (CyclinD1, CyclinE) and cyclin-dependent kinase (CDK2, CDK4);6 Shiquandabu Tang Granules could promote gene expression of EPOmRAN in Kidney and BMC, TPOmRNA in spleen, GM-CSFmRNA in BMC, increase the level of serum EPO, TPO and GM-CSF;.7 Shiquandabu Tang Granules could promote early hematopoietic stem/progenitor cells adhesion and homing by increasing the adhesion of bone marrow stromal cells, promoting the expression of FN, LN, and inhibiting the expression of MMP-2, MMP-7, MMP-9 in extracellular matrix of bone marrow.Conclusion:Within a certain dose range, Shiquandabu Tang Granules could promote effectively the recovery of impaired hematopoietic function in myelosuppressed mice. The main channels may be regulating cell cycle of BMC, improving the bone marrow micro-environment, promoting the gene expression of hematopoietic growth factors. There has no significant difference between the Shiquandabu Tang Granules and the traditional decoction in improving the bone marrow hematopoietic function of myelosuppressed mice.