Cadherin: As A Receptor For Adhesion And Endocytosis Of Aspergillus Fumigatus By A549 And HUVEC

Aspergillus fumigatus is an ubiquitous sapropHytic fungus responsible for the majority of invasive mold infections. Airborne conidia (spores) are inhaled into the small airways, where those escape from pHagocytose of pulmonary pHagocytic cells may germinate and initiate an infection. The invasion of airway epithelial cells is therefore a key step in the etiology of aspergillosis, but little is known of the factors involved in this interaction.Many receptors including adhesion molecules and pattern recognition receptors are the promising candidates. Pattern recognition receptors and the associated adaptor molecules play a role in mediating the inflammatory response to A.fumigatus, but few research indicated that they also could get involved in invasion. Cadherin belong to adhensive molecule familys serves as an endothelial cell receptor, which mediates the endocytosis of C. albicans. The listerial protein internalin (InlA) mediate Listerial adhesion and invasion of epithelial cell through the exact interreaction with the receptor cadherin. N-terminal domain of cadherin is recognized and engulfed by InlA with its'leucine rich repeat (LRR) domain. We have found that there are some homologous or similar molecules to InlA in Aspergillus fumigatus through BLASTp in bioinformatics.Could e-cadherin (epithelial type cadherin) be a receptor for adhesion and endocytosis of Aspergillus fumigatus blastospores.Partâ… E-cadherin:As a receptor for adhesion and endocytosis of Aspergillus fumigatus blastospores by A549Objective:To study the receptor for adhesion and endocytosis of Aspergillus fumigatus blastospores by A549. Methods:Aspergillus fumigatus blastospores were incubated with the total protein of A549 for investigating the binding of e-cadherin and blastospore. After establishing the model of adhesion and endocytosis of Aspergillus fumigatus blastospores by A549, the capacity of adherion and endocytosis was evaluated with the existence of the antibody for e-cadherin. Result:E-cadherin sticked to the surface of Aspergillus fumigatus blastospore. Adherion and endocytosis was weaken with the existence of the antibody for e-cadherin. Conclusion:E-cadherin is a receptor for adhesion and endocytosis of Aspergillus fumigatus blastospores by A549.Partâ…¡N-cadherin:As a receptor for adhesion and endocytosis of Aspergillus fumigatus by HUVEC Objective:To evaluate role of n-cadherin as the receptor for adhesion and endocytosis of Aspergillus fumigatus hypHae by HUVEC. Methods:Aspergillus fumigatus hypHae were incubated with the total protein of HUVEC for fingding out the binding of e-cadherin and Aspergillus fumigatus hypHae. After establishing the model of adhesion and endocytosis of Aspergillus fumigatus hypHae by HUVEC, the amount of hypHae of adherion and endocytosis was recorded with the existence of the antibody for N-cadherin. Result: N-cadherin sticked to the surface of Aspergillus fumigatus hypHae. The amount of hypHae of adherion and endocytosis decreased with the existence of the antibody for N-cadherin. Conclusion:N-cadherin is a receptor for adhesion and endocytosis of Aspergillus fumigatus hypHae by HUVEC.