Expression And Mechanism Of Action Of Chemokine Receptor In Hepatoma Carcinoma Cell

Objective:Hepatocellular carcinoma (HCC) is the fifth deadly tumor in the world, Development of HCC is associated with hepatic B virus. HCC patients do not have specifity of symptom, so many patients lost opportunity to operate. Non-surgery therapies such as transaterial chemotherapy embolization (TACE), radiofrequency ablation (RFA), percutaneous ethanol injection (TEI) has limited effects. Many patients succumb to the recurrence and metastasis at last. Biotherapy and gene therapysuppose the new direction for therapy of metastasis of HCC. However, invasion and metastasis of HCC is a process of multi-step and multi-gene, which is concerned to biological characteristics oftumor cells, the local microenviroments in tumor such as matrix metalloprotease (MMP), integrin, stroma-cell and blood vessel, and local and systematic immunology. Chemokines and chemokine receptors are the critical molecular in homing and directionalchemotaxis of immunocells. To date, about 45 chemokines and 18 chemokine receptors have thedefinite functions. Another chemokine and chemokine receptors have not clear in function. In2001, Muller first reported the expressions of chemokine receptors in breast cancer were related tothe metastasis of tumor cells, particularly CXCR4 in lung metastasis, and CCR7 in lymph nodemetastasis. By now, more and more researchers have shown that chemokine receptors can befunctionally expressed in tumor cells, particularly in tumor cells with high metastasis potentials.Therefore, we establlish the relationship between HCC and chemokoine receptor. To investigate the expression and mechanism of chemokine receptor (CCR1 CCR7 CXCR3 CXCR4) in hepatoma carcinoma cell (Hep3B HepG2 HLE and HLF)Methods:RT-PCR immunohistochemistry and Flow Cytometric were used to determine the expression and mechanism of CCRl chemokine receptor in hepatoma carcinoma cell. invasion of Hep3B was analyzed with basal membrane by CCL3 and mechanism of chemokine receptor was analyzed in hepatoma carcinoma cell by confocal microscopy,Result:CCR1 mRNA and protein could be detected in hepatoma carcinoma cell. Immunohistochemistry revealed that CCR1 protein was located on membrane and intracytoplasm of hepatoma carcinoma cell. The number of hepatoma carcinoma cells that go through basal membrane were significantly more in experiment group(213±12) than the number of hepatoma carcinoma cells were in control group (102±11) (P<0.01). and the density of the calciumion increases in hepatoma carcinoma cells in experiment group.Conclusions:hepatoma carcinoma cell expresses CCR1 chemokine recepter, and CCR1 plays an important role in course of metastasis and invasion of hepatoma carcinoma cell. and the density of the calciumion have a great relationship with the mechanism of chemokine receptor in hepatoma carcinoma.