| Background: With the development of social economic and the change of people's lifestyles, the incidence of angina pectoris increases year by year, and become one of the main diseases which impact on the life expectancy and quality of life. Therefore, it is of great importance on social meaning and health economics meaning to research the disease of angina pectoris. Traditional Chinese medicine plays a unique value in treating chronic stable angina, but the law of syndrome, generation mechanism prognostic value needs to be further researched to give full play to TCM and to provide basis for the syndrome differentiation and clinical diagnosis of angina pectoris.Objective:To discuss the relationship between common clinical manifestation and related serum protein expression in angina pectoris.Methods:1. Clinical syndrome in patients with coronary stable angina cordis: patients with coronary stable angina cordis proved by coronal artery angiography were analyzed the characters of clinical syndrome. The character included syndrome elements, the common syndrome distribution, the risk factor between common syndrome and syndrome elements, the differences of coronary angiographic degree. The relative model was built by using MDR, and then calculated the relationship between clinical manifestation and syndrome of patients with angina pectoris.2. The relationship between protein expression and syndrome in patients with coronary stable angina cordis:the content of Se-Selectin, sVCAM-1, sICAM-1, MMP-9, MPO, tPAI-1 was detected by analysis of liquid protein chip, statisticed the protein differential expressions in syndrome elements and common syndrome, researched the relationship among syndrome elements, common syndrome and protein expression.Results:1.The distribution of common syndrome elements in patients with coronary stable angina cordis shows that 147 cases of phlegm,145 cases of stasis,115 cases of qi deficiency,73 cases of yin deficiency,14 cases of qi stagnation,24 cases of yang deficiency,22 cases of heat,4 cases of cold. The common syndrome shows that 85 cases of qi deficiency and blood stasis,92 cases of phlegm and blood stasis,56 cases of yin deficiency and phlegm obstruction,86 cases of qi deficiency and phlegm obstruction,42 cases of both qi and yin deficiency and phlegm obstruction,21 cases of both qi and yin deficiency and phlegm stasis. Compare with different syndrome, there is no statistically significance in risk factor and coronary angiographic degree.2. The characteristic of syndrome elements and common syndrome in patients with angina pectoris:for cold (dizziness, palpitation, depression, the shape of choroids) the accuracy was 0.884, sensitivity was 1.000, specifty was 0.881 and the predicative accuracy was 0.143; for qi deficiency (pale complexion, stabbing pain in the chest, cyanotic lips and tongue, soreness) the accuracy was 0.718, sensitivity was 0.722, specifty was0.741 and the predicative accuracy was 0.762; for qi stagnation, (preference for sighing, stabbing pain in the chest, edema of both lower limbs, purplish grayish tongue) the accuracy was 0.786, sensitivity was 1, specifty was0.771and the predicative accuracy was 0.241; for heat(palpitation, cyanotic lips and tongue, the color of sublingual vein, abdominal distension) the accuracy was 0.704, sensitivity was 0.909, specifty was0.679and the predicative accuracy was 0.253; for phlegm(abdominal distension, palpitation, the color of sublingual vein, cyanotic lips and tongue) the accuracy was 0.762, sensitivity was 0.748, specifty was0.797and the predicative accuracy was 0.902;for yang deficiency(abdominal distension, the color of sublingual vein, cyanotic lips and tongue) the accuracy was 0.704, sensitivity was 0.875, specifty was0.681and the predicative accuracy was 0.266;for yin deficiency(abdominal distension, palpitation, the color of sublingual vein, cyanotic lips and tongue) the accuracy was 0.728, sensitivity was 0.795, specifty was0.692and the predicative accuracy was 0.586; for blood stasis(abdominal distension, palpitation, the color of sublingual vein, cyanotic lips and tongue) the accuracy was 0.7476, sensitivity was 0.731, specifty was0.7869and the predicative accuracy was 0.8908; for qi deficiency and blood stasis (constipation, palpitation, the shape of choroids, fat body) the accuracy was 0.8204, sensitivity was 0.8947, specifty was0.8128and the predicative accuracy was 0.3269;for phlegm and blood stasis(constipation, palpitation, the shape of choroids, fat body) the accuracy was 0.7718, sensitivity was 1, specifty was0.759and the predicative accuracy was 0.1897; for yin deficiency and phlegm obstruction(constipation, palpitation, the shape of choroids, fat body) the accuracy was 0.825, sensitivity was 1.000, specifty was0.818, the predicative accuracy was 0.182; for qi deficiency and phlegm obstruction(dyspnea chest discomfort, buring sensation of five centers, preference for sighing,depression) the accuracy was 0.898, sensitivity was 1.000, specifty was 0.895 and the predicative accuracy was 0.250; for qi stagnation and blood stasis(dyspnea chest discomfort, buring sensation of five centers, preference for sighing,depression) the accuracy was 0.854, sensitivity was 1.000, specifty was 0.849 and the predicative accuracy was 0.189;for yin-asthenia and blood-stasis(palpitation, overweight and phlegmatic, constipation, thread of thought) the accuracy was 0.854, sensitivity was 1.000, specifty was 0.849 and the predicative accuracy was 0.189;for yang deficiency and phlegm obstruction(palpitation, overweight and phlegmatic, constipation, the shape of choroids) the accuracy was 0.864, sensitivity was 1.000, specifty was 0.859 and the predicative accuracy was 0.200;for qi deficiency and blood stasis and phlegm obstruction(abdominal distension, palpitation, the color of sublingual vein, cyanotic lips and tongue) the accuracy was 0.7476, sensitivity was 0.8529, specifty was 0.7267 and the predicative accuracy was 0.3816;for both of qi and yin deficiency and phlegm obstruction(palpitation, overweight and phlegmatic, constipation, the shape of choroids) the accuracy was 0.830, sensitivity was 1.000, specifty was 0.816 and the predicative accuracy was 0.314;for both of deficiency and phlegm stasis(overweight and phlegmatic, dyspnea chest discomfort, whitish tongue fur and pale complexion) the accuracy was 0.811, sensitivity was 0.950, specifty was 0.796 and the predicative accuracy was 0.333.3. The protein expression in sVCAM-1, sICAM-1, MMP-9, MPO, tPAI-1 in patients with coronary stable angina cordis is higher than health(P<0.01). There is no statistically meaning in sE-Selectin in both groups (p=0.067) 4. Protein expression of syndrome elements in patients with coronary stable angina cordis:The expression of tPAI-1 is higher in phlegm, stasis, qi deficiency, yin deficiency, heat; The expression of sVCAM-1 is higher in phlegm, stasis, qi deficiency, qi stagnation, yin deficiency, heat; the expression of sICAM-1 is higher in phlegm, stasis, qi deficiency, qi stagnation, yin deficiency, heat; The expression of MMP-9 is higher in phlegm, stasis, qi deficiency, qi stagnation, yin deficiency, heat; the expression of Se-Selectin is higher in phlegm, stasis, qi deficiency, yin deficiency; The expression of MPO is higher in phlegm, stasis, qi deficiency, qi stagnation, yang deficiency, heat.5. Protein expression of syndrome in patients with coronary stable angina cordis:The expression of tPAI-1 is higher in qi deficiency and blood stasis, phlegm and blood stasis, yin deficiency and phlegm obstruction, qi deficiency and phlegm obstruction, yin deficiency and blood stasis; qi deficiency and blood stasis and phlegm stagnation, both qi and yin deficiency and phlegm obstruction, both qi and yin deficiency and phlegm stasis (P<0.01); The expression of sVCAM-1 is higher in qi deficiency and blood stasis, phlegm and blood stasis, yin deficiency and phlegm obstruction, qi deficiency and phlegm obstruction, yin deficiency and blood stasis, qi deficiency and blood stasis and phlegm stagnation, both qi and yin deficiency and phlegm obstruction, both qi and yin deficiency and phlegm stasis (P<0.01); The expression of sICAM-1 is higher in qi deficiency and blood stasis, phlegm and blood stasis, yin deficiency and phlegm obstruction, qi deficiency and phlegm obstruction, yin deficiency and blood stasis, qi deficiency and blood stasis and phlegm stagnation, both qi and yin deficiency and phlegm obstruction, both qi and yin deficiency and phlegm stasis (P<0.01); The expression of MMP-9 is higher in qi deficiency and blood stasis, phlegm and blood stasis, phlegm and blood stasis, qi deficiency and phlegm obstruction, qi stagnation and blood stasis, qi deficiency and blood stasis and phlegm stagnation, both qi and yin deficiency and phlegm obstruction, both qi and yin deficiency and phlegm stasis (P<0.01or 0.05); The expression of Se-Selectin is higher in qi deficiency and blood stasis, phlegm and blood stasis, qi deficiency and phlegm obstruction, yin deficiency and phlegm obstruction, qi deficiency and blood stasis and phlegm stagnation, both qi and yin deficiency and phlegm obstruction, both qi and yin deficiency and phlegm stasis (P<0.01); The expression of Se-Selectin is higher than health and yang deficiency; The expression of MPO is higher in qi deficiency and blood stasis, phlegm and blood stasis, qi deficiency and phlegm obstruction, yin deficiency and phlegm obstruction, yin deficiency and blood stasis, qi deficiency and blood stasis and phlegm stagnation, both qi and yin deficiency and phlegm obstruction, both qi and yin deficiency and phlegm stasis (P<0.01);Conclusion:1.The common syndrome elements in patients with coronary stable angina cordis can be seen as phlegm, stasis, qi deficiency, yin deficiency, qi stagnation, yang deficiency, heat, cold; while the common syndrome can be seen as qi deficiency and blood stasis, phlegm and blood stasis, qi deficiency and phlegm obstruction, qi stagnation and blood stasis, yin deficiency and blood stasis, yang deficiency and phlegm obstruction, qi deficiency and blood stasis and phlegm obstruction, both of qi and yin deficiency and phlegm obstruction, both of qi and yin deficiency and phlegm stasis.2.The clinical manifestation of syndrome elements in patients with coronary stable angina cordis can be summarized as following: cold(dizziness, palpitation, depression, stabbing pain in the chest, the shape of choroids); qi deficiency(pale complexion, stabbing pain in the chest, cyanotic lips and tongue, sorness); qi stagnation(preference for sighing, stabbing pain in the chest, edema of both lower limbs, purplish grayish tongue);heat (palpitation, cyanotic lips and tongue, the color of sublingual vein, abdominal distension); phlegm(abdominal distension, palpitation, the color of sublingual vein, cyanotic lips and tongue);yang deficiency(abdominal distension, palpitation, the color of sublingual vein, cyanotic lips and tongue);yin deficiency (abdominal distension, palpitation, the color of sublingual vein, cyanotic lips and tongue);blood stasis(abdominal distension, palpitation, the color of sublingual vein, cyanotic lips and tongue);3. The clinical manifestation of syndrome elements in patients with coronary stable angina cordis can be summarized as following: qi deficiency and blood stasis(constipation, fat body, palpitation, the shape of choroids); phlegm and blood stasis(constipation, fat body, palpitation, the shape of choroids);yin deficiency and phlegm obstruction(constipation, fat body, palpitation, the shape of choroids); yin deficiency and blood stasis(constipation, fat body, palpitation, the shape of choroids); qi deficiency and blood stasis and phlegm obstruction(abdominal distension, palpitation, the color of sublingual vein, cyanotic lips and tongue); both of qi and yin deficiency and phlegm obstruction(abdominal distension, palpitation, the color of sublingual vein, cyanotic lips and tongue, constipation); both of qi and yin deficiency and phlegm stasis(fat body, stabbing pain in the chest, pale complexion, pale tongue).4. The content of sVCAM-1, sICAM-1, MMP-9, MPO, tPAI-1 in patients with coronary stable angina cordis is higher than health.5. The expression of tPAI-1, sVCAM-1, sICAM-1, sE-Selectin, MMP-9, MPO, in phlegm, stasis, qi deficiency, yin deficiency and heat increase higher; while the expression of sVCAM-1,MMP-9, MPO in qi stagnation increased significantly; MPO in yang deficiency increased.6.The expression of sVCAM-1, sICAM-1, MMP-9, MPO, tPAI-1,sE-Selectin in qi deficiency and blood stasis, phlegm and blood stasis, yin deficiency and phlegm obstruction, qi deficiency and phlegm obstruction, yin deficiency an blood stasis increased; while the expression of sVCAM-1, sICAM-1, MMP-9, MPO in qi stagnation and blood stasis increased higher; the expression of sVCAM-1, sICAM-1, MMP-9, MPO, tPAI-1,sE-Selectin in qi deficiency and blood stasis and phlegm obstruction, both of qi and yin deficiency and phlegm stasis increases higher.
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